Molykutty J, Schultz G, Rajalekshmy T N, Enose S, Nair M K, Balaram P
Regional Cancer Centre, Medical College P.O., Trivandrum, Kerala, India.
Tumori. 1999 May-Jun;85(3):183-7. doi: 10.1177/030089169908500307.
Complete hydatidiform mole (CHM), a condition related to abnormal gestation, occurs predominantly in the young reproductive age group and has a high prevalence rate in the Trivandrum region, occurring in 1.2% of deliveries. Transforming growth factor alpha (TGF-alpha) is an important growth regulatory molecule, the location and function of which at the human fetomaternal interface in CHM remains to be determined. The present study examined the presence of TGF-alpha in the normal and complete molar placenta and decidua throughout gestation. A total of 149 complete molar placental tissue samples and 96 normal placental tissue samples were evaluated for TGF-alpha expression by immunohistochemistry and 50 each of CHM and normal placental tissue for TGF-alpha concentration by radioimmunoassay. The peptide was localized immunocytochemically with a monoclonal anti-TGF-alpha antibody on paraffin-embedded tissue using the avidin-biotin complex peroxidase technique with aminoethyl carbazole as the chromogen. In molar placenta, villous trophoblast cells (syncytiotrophoblasts and cytotrophoblasts) showed intense cytoplasmic staining at all gestational ages compared to normal placenta of the same gestational age. The tissue concentration of TGF-alpha was highly overexpressed in the molar placenta (10-1000 fold) compared to that in the normal placenta. The results indicate that TGF-alpha is present in trophoblasts throughout human gestation and may provide additional growth advantage to maintenance of the hyperproliferative condition in trophoblastic tumors.
完全性葡萄胎（CHM）是一种与异常妊娠相关的疾病，主要发生在年轻育龄人群中，在特里凡得琅地区发病率较高，占分娩总数的1.2%。转化生长因子α（TGF-α）是一种重要的生长调节分子，其在CHM患者母胎界面的定位和功能尚待确定。本研究检测了整个孕期正常及完全性葡萄胎胎盘和蜕膜中TGF-α的存在情况。通过免疫组织化学对149份完全性葡萄胎胎盘组织样本和96份正常胎盘组织样本进行TGF-α表达评估，通过放射免疫分析对50份完全性葡萄胎胎盘组织样本和50份正常胎盘组织样本进行TGF-α浓度检测。采用抗生物素蛋白-生物素复合物过氧化物酶技术，以氨基乙基咔唑为显色剂，用抗TGF-α单克隆抗体对石蜡包埋组织进行免疫细胞化学定位。与相同孕周的正常胎盘相比，在葡萄胎胎盘中，绒毛滋养层细胞（合体滋养层细胞和细胞滋养层细胞）在所有孕周均显示出强烈的细胞质染色。与正常胎盘相比，葡萄胎胎盘中TGF-α的组织浓度高度过表达（10 - 1000倍）。结果表明，TGF-α在人类整个孕期的滋养层细胞中均有表达，可能为滋养层肿瘤维持过度增殖状态提供额外的生长优势。
