用于微孔板形式酵母的原生质球裂解试验。
The spheroplast lysis assay for yeast in microtiter plate format.
作者信息
Ovalle R, Spencer M, Thiwanont M, Lipke P N
机构信息
Department of Biological Sciences and Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, New York, New York 10021, USA.
出版信息
Appl Environ Microbiol. 1999 Aug;65(8):3325-7. doi: 10.1128/AEM.65.8.3325-3327.1999.
Abstract
A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for drug susceptibility and for analyzing mutant phenotypes.
摘要
微量滴定板形式的酵母裂解试验提高了精度和通量，并产生了一种改进的估算延迟时间的算法。该试验可重复性地揭示出最大裂解率差异达10%或更大，延迟时间差异达50%或更大。克隆差异被确定为主要变异来源。基于微量滴定板的试验对于筛选药物敏感性和分析突变体表型应该是有用的。
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