Lussier M, White A M, Sheraton J, di Paolo T, Treadwell J, Southard S B, Horenstein C I, Chen-Weiner J, Ram A F, Kapteyn J C, Roemer T W, Vo D H, Bondoc D C, Hall J, Zhong W W, Sdicu A M, Davies J, Klis F M, Robbins P W, Bussey H
Department of Biology, McGill University, Montréal, Québec, Canada.
Genetics. 1997 Oct;147(2):435-50. doi: 10.1093/genetics/147.2.435.
The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes involved in cell surface assembly by screening transposon-mutagenized cells for altered sensitivity to calcofluor white, followed by supplementary screens to further characterize mutant phenotypes. The mutated genes were directly retrieved from genomic DNA and then matched uniquely to a gene in the yeast genome database. Eighty-two genes with apparent perturbation of the cell surface were identified, with mutations in 65 of them displaying at least one further cell surface phenotype in addition to their modified sensitivity to calcofluor. Fifty of these genes were previously known, 17 encoded proteins whose function could be anticipated through sequence homology or previously recognized phenotypes and 15 genes had no previously known phenotype.
测序的酵母基因组为真核细胞功能分析提供了独特的资源，并能够对参与细胞过程的基因进行全基因组筛选。我们通过筛选转座子诱变细胞对荧光增白剂的敏感性变化来鉴定参与细胞表面组装的基因，随后进行补充筛选以进一步表征突变体表型。突变基因直接从基因组DNA中获取，然后与酵母基因组数据库中的基因进行唯一匹配。我们鉴定出了82个明显影响细胞表面的基因，其中65个基因的突变除了对荧光增白剂的敏感性改变外，还表现出至少一种其他细胞表面表型。这些基因中有50个是先前已知的，17个编码的蛋白质其功能可通过序列同源性或先前识别的表型预测，还有15个基因以前没有已知的表型。
