源自非肥胖糖尿病（NOD）小鼠的胚胎干细胞（ES）系的建立：体内分化为淋巴细胞及种系传递潜能
Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: in vivo differentiation into lymphocytes and potential for germ line transmission.
作者信息
Nagafuchi S, Katsuta H, Kogawa K, Akashi T, Kondo S, Sakai Y, Tsukiyama T, Kitamura D, Niho Y, Watanabe T
机构信息
First Department of Internal Medicine, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
出版信息
FEBS Lett. 1999 Jul 16;455(1-2):101-4. doi: 10.1016/s0014-5793(99)00801-7.
A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(beta)g7 haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established.
一种源自非肥胖型糖尿病（NOD）小鼠的胚胎干细胞（ES）系已稳定维持在未分化状态，具有典型的ES细胞样形态，表达干细胞标志物碱性磷酸酶，并呈现正常的二倍体核型。将NOD-ES细胞注射到囊胚中后，获得了嵌合小鼠。少量但有显著数量的淋巴细胞表达源自NOD的MHC等位基因。当一只嵌合小鼠与C57BL/6小鼠交配时，获得了一只具有源自NOD的H-2 I-A(beta)g7单倍型的刺鼠。因此，成功建立了一种能够分化为具有种系传递潜力的淋巴细胞的NOD-ES细胞系。
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