Suzuki H, Kamada N, Ueda O, Jishage K, Kurihara Y, Kurihara H, Terauchi Y, Azuma S, Kadowaki T, Kodama T, Yazaki Y, Toyoda Y
Laboratory of Molecular Genetics and Embryology, CSK Research Park Inc., Tokyo, Japan.
Exp Anim. 1997 Jan;46(1):17-23. doi: 10.1538/expanim.46.17.
The effect of the karyotype and the ability to differentiate in vitro upon germ-line transmission by A3-1 embryonic stem (ES) cells in chimeric mice were examined. Germ-line transmission was confirmed in ES cells exhibiting 38% and more of the normal karyotype, but no chimeric mice and/or germ-line transmitters were observed regardless of the karyotype when the cystic embryoid body (CEB) was formed on day 8 and later in the suspension culture. Germ-line transmission of the ES cells was not significantly influenced by formation of the simple embryoid body (SEB). Germ-line transmitters were preferentially observed in chimeras when the ES cell contribution to coat color was markedly increased, but this contribution to coat color varied regardless of the karyotype or in vitro differentiation ability. These results suggest that A3-1 ES cells which exhibit CEB at 7 days after suspension culture and approximately 40% of normal karyotype are capable of germ-line transmission in chimeric mice.
研究了A3-1胚胎干细胞（ES细胞）的核型以及体外分化能力对嵌合小鼠种系传递的影响。在核型正常率达38%及以上的ES细胞中证实了种系传递，但当在悬浮培养第8天及以后形成囊性胚状体（CEB）时，无论核型如何，均未观察到嵌合小鼠和/或种系传递者。简单胚状体（SEB）的形成对ES细胞的种系传递没有显著影响。当ES细胞对毛色的贡献显著增加时，在嵌合体中优先观察到种系传递者，但这种对毛色的贡献无论核型或体外分化能力如何都有所不同。这些结果表明，悬浮培养7天后出现CEB且核型约为正常40%的A3-1 ES细胞能够在嵌合小鼠中实现种系传递。
