Kawazu S, Kamio T, Kakuda T, Terada Y, Sugimoto C, Fujisaki K
National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
Int J Parasitol. 1999 Apr;29(4):613-8. doi: 10.1016/s0020-7519(99)00008-9.
In order to examine the taxonomic relationship of Theileria sp. of Asian buffalo to the benign Theileria spp. of cattle, we sequenced and compared the major piroplasm protein (p33/34) genes of these parasites. The two consensus sequences determined for the buffalo parasite were of the same length (852 bp) and showed >80% identity with the sequences of the homologous genes (849 bp) in the cattle parasites. Alignment of the inferred aa sequences with those of Theileria sergenti and Theileria buffeli predicted that there is an insertion of a single residue at the N-terminus in the inferred polypeptide of the buffalo parasite. Phylogenetic analyses based on the aa sequences suggested that Theileria sp. of the Asian buffalo should be classified within the benign Theileria parasite group as a separate species from the cattle parasites. Based on this, we propose a rearrangement of the currently used classification for the benign Theileria species in cattle and Asian buffalo.
为了研究亚洲水牛泰勒虫与牛的良性泰勒虫种类之间的分类关系，我们对这些寄生虫的主要梨形虫蛋白（p33/34）基因进行了测序和比较。水牛寄生虫的两个共有序列长度相同（852 bp），并且与牛寄生虫中同源基因（849 bp）的序列具有>80%的同一性。将推断的氨基酸序列与瑟氏泰勒虫和水牛泰勒虫的序列进行比对，预测水牛寄生虫推断的多肽在N端有一个单残基插入。基于氨基酸序列的系统发育分析表明，亚洲水牛泰勒虫应归类于良性泰勒虫寄生虫组内，作为与牛寄生虫不同的一个单独物种。基于此，我们建议对目前用于牛和亚洲水牛良性泰勒虫种类的分类进行重新排列。
