Chapman A P, Antoniw P, Spitali M, West S, Stephens S, King D J
Celltech Therapeutics, 216 Bath Rd., Slough, Berks SL1 4EN, UK.
Nat Biotechnol. 1999 Aug;17(8):780-3. doi: 10.1038/11717.
Antibody fragments can be isolated rapidly using techniques such as phage display and can be expressed to high levels in microbial systems. However, to date such antibody fragments have been of limited use for many therapeutic applications because they are rapidly cleared from the body. We present a strategy for the site-specific chemical modification of antibody fragments with polyethylene glycol, which results in the production of antibody fragments with long in vivo half-lives and full retention of antigen-binding properties. This technology should allow more rapid and economical production of therapeutic antibodies for chronic disease therapy.
使用噬菌体展示等技术可以快速分离抗体片段，并且它们能够在微生物系统中高水平表达。然而，迄今为止，此类抗体片段在许多治疗应用中的用途有限，因为它们会迅速从体内清除。我们提出了一种用聚乙二醇对抗体片段进行位点特异性化学修饰的策略，该策略可产生具有长体内半衰期且完全保留抗原结合特性的抗体片段。这项技术应能更快速、经济地生产用于慢性病治疗的治疗性抗体。
