Cdc45p与DNA聚合酶在DNA复制早期和晚期起始位点的差异组装。
Differential assembly of Cdc45p and DNA polymerases at early and late origins of DNA replication.
作者信息
Aparicio O M, Stout A M, Bell S P
机构信息
Massachusetts Institute of Technology, Department of Biology, Room 68-622, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9130-5. doi: 10.1073/pnas.96.16.9130.
Abstract
Chromosomes are replicated in characteristic, temporal patterns during S phase. We have compared the timing of association of replication proteins at early- and late-replicating origins of replication. Minichromosome maintenance proteins assemble simultaneously at early- and late-replicating origins. In contrast, Cdc45p association with late origins is delayed relative to early origins. DNA polymerase alpha association is similarly delayed at late origins and requires Cdc45p function. Activation of the S phase checkpoint inhibits association of Cdc45p with late-firing origins. These studies suggest that Cdc45p is poised to serve as a key regulatory target for both the temporal and checkpoint-mediated regulation of replication origins.
摘要
染色体在S期按照特定的时间模式进行复制。我们比较了复制蛋白在早期和晚期复制起始点的结合时间。微小染色体维持蛋白在早期和晚期复制起始点同时组装。相比之下，Cdc45p与晚期起始点的结合相对于早期起始点有所延迟。DNA聚合酶α在晚期起始点的结合同样延迟，且需要Cdc45p发挥功能。S期检查点的激活会抑制Cdc45p与晚期起始点的结合。这些研究表明，Cdc45p有望成为复制起始点时间调控和检查点介导调控的关键调控靶点。
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