手部清洁制剂清除污染手部鲍曼不动杆菌菌株的有效性。

Effectiveness of hand-cleansing agents for removing Acinetobacter baumannii strain from contaminated hands.

作者信息

Cardoso C L, Pereira H H, Zequim J C, Guilhermetti M

机构信息

Department of Clinical Analyses, Laboratory of Microbiology, State University of Maringá, Paraná, Brazil.

出版信息

Am J Infect Control. 1999 Aug;27(4):327-31. doi: 10.1016/s0196-6553(99)70052-0.

DOI:10.1016/s0196-6553(99)70052-0

PMID:10433671

Abstract

BACKGROUND

The effectiveness of hand-cleansing agents (plain liquid soap, 70% ethyl alcohol, 10% povidone-iodine, and 4% chlorhexidine gluconate) for removing a hospital strain of Acinetobacter baumannii from artificially contaminated hands of 5 volunteers was studied.

METHODS

The experiments were performed by using a Latin square statistical design, with two 5 x 4 randomized blocks, and the results were estimated by ANOVA. In the first and second blocks, the fingertips of the volunteers were contaminated with approximately 10(3) colony-forming units (light contamination hand) and 10(6) colony-forming units (heavy contamination hand), respectively.

RESULTS

In the first block, all products tested were effective, almost completely removing the microbial population of A baumannii artificially applied to the hands. In the second block, the use of hand-cleansing agents resulted in 91.36% (4% chlorhexidine), 92.33% (liquid soap), 98.49% (10% povidone-iodine), and 98.93% (70% ethyl alcohol) reduction in counts of A baumannii cells applied to the fingertips. The ethyl alcohol and povidone-iodine had significantly higher removal rates than plain soap and chlorhexidine (P <.05).

CONCLUSIONS

These results suggest that 70% ethyl alcohol and 10% povidone-iodine may be the most effective hand-cleansing agents for removing A baumannii strain from heavily contaminated hands (10(6) colony-forming units/fingertip).

摘要

背景

研究了手部清洁制剂（普通液体肥皂、70%乙醇、10%聚维酮碘和4%葡萄糖酸氯己定）对5名志愿者人工污染手部的医院鲍曼不动杆菌菌株的清除效果。

方法

实验采用拉丁方统计设计，有两个5×4随机区组，结果通过方差分析进行评估。在第一个和第二个区组中，志愿者的指尖分别被约10³菌落形成单位（轻度污染手）和10⁶菌落形成单位（重度污染手）污染。

结果

在第一个区组中，所有测试产品均有效，几乎完全清除了人工接种到手上的鲍曼不动杆菌微生物群落。在第二个区组中，使用手部清洁制剂后，接种到指尖的鲍曼不动杆菌细胞计数减少了91.36%（4%葡萄糖酸氯己定）、92.33%（液体肥皂）、98.49%（10%聚维酮碘）和98.93%（70%乙醇）。乙醇和聚维酮碘的清除率明显高于普通肥皂和氯己定（P<.05）。