Yaqoob P, Newsholme E A, Calder P C
Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
Cytokine. 1999 Aug;11(8):600-5. doi: 10.1006/cyto.1998.0471.
In order to examine inter- and intra-individual variations in cytokine production, blood was collected from 48 healthy subjects on each of 4 occasions separated by 4 weeks. Whole blood (diluted 1:10) and mononuclear cell (MNC) cultures were stimulated for 24 h with either concanavalin A (Con A) or bacterial lipopolysaccharide (LPS) and the concentrations of IL-1alpha, IL-1beta, IL-2, TNF-alpha, IL-10 and IFN-gamma in the culture medium measured. There were highly significant inter-individual variations in the production of each of the cytokines measured. However, the level of the production of each cytokine appeared to be characteristic of an individual. There were significant correlations between production of each cytokine in whole blood and MNC cultures. It is concluded that there is significant inter-individual variation in cytokine production which is unaffected by time or by the stimulus used to elicit cytokine production, and that whole blood cultures can be used instead of MNC cultures to measure cytokine production.
为了检测细胞因子产生的个体间和个体内差异，在间隔4周的4个不同时间点，从48名健康受试者采集血液。用伴刀豆球蛋白A（Con A）或细菌脂多糖（LPS）刺激全血（稀释1:10）和单核细胞（MNC）培养物24小时，并测量培养基中IL-1α、IL-1β、IL-2、TNF-α、IL-10和IFN-γ的浓度。在所检测的每种细胞因子的产生方面，个体间存在高度显著差异。然而，每种细胞因子的产生水平似乎是个体特有的。全血和MNC培养物中每种细胞因子的产生之间存在显著相关性。得出的结论是，细胞因子产生存在显著的个体间差异，不受时间或用于引发细胞因子产生的刺激的影响，并且全血培养物可用于替代MNC培养物来测量细胞因子产生。
