Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading RG6 6AP, UK.
Nutr Res. 2017 Oct;46:88-95. doi: 10.1016/j.nutres.2017.09.006. Epub 2017 Sep 21.
This study hypothesized that the predominant strawberry anthocyanin, pelargonidin-3-O-glucoside (Pg-3-glc), and 3 of its plasma metabolites (4-hydroxybenzoic acid, protocatechuic acid, and phloroglucinaldehyde [PGA]) would affect phagocytosis, oxidative burst, and the production of selected pro- and anti-inflammatory cytokines in a whole blood culture model. For the assessment of phagocytosis and oxidative burst activity of monocytes and neutrophils, whole blood was preincubated in the presence or absence of the test compounds at concentrations up to 5 μmol/L, followed by analysis of phagocytic and oxidative burst activity using commercially available test kits. For the cytokine analysis, diluted whole blood was stimulated with lipopolysaccharide in the presence or absence of the test compounds at concentrations up to 5 μmol/L. Concentrations of selected cytokines (tumor necrosis factor-α, interleukin [IL]-1β, IL-6, IL-8, and IL-10) were determined using a cytometric bead array kit. There were no effects of any of the test compounds on phagocytosis of opsonized or nonopsonized Escherichia coli or on oxidative burst activity. Pg-3-glc and PGA at 0.08 μmol/L increased the concentration of IL-10 (P<.01 and P<.001, respectively), but there was no effect on tumor necrosis factor-α, IL-1β, IL-6, and IL-8, and there were no effects of the other compounds. In conclusion, this study demonstrated a lack of effect of these compounds on the opsonization, engulfment, and subsequent destruction of bacteria. Pg-3-glc and PGA, at physiologically relevant concentrations, had anti-inflammatory properties; however, effects were modest, only observed at the lowest dose tested and limited to IL-10.
本研究假设，主要的草莓花色苷矢车菊素-3-O-葡萄糖苷（Pg-3-glc）及其 3 种血浆代谢物（4-羟基苯甲酸、原儿茶酸和根皮醛[PGA]）将影响吞噬作用、氧化爆发以及全血培养模型中选定的促炎和抗炎细胞因子的产生。为了评估单核细胞和中性粒细胞的吞噬作用和氧化爆发活性，全血在存在或不存在测试化合物的情况下于高达 5μmol/L 的浓度下进行预孵育，然后使用市售的测试试剂盒分析吞噬作用和氧化爆发活性。为了进行细胞因子分析，用脂多糖刺激稀释的全血，同时在高达 5μmol/L 的浓度下存在或不存在测试化合物。使用流式细胞术检测试剂盒测定选定细胞因子（肿瘤坏死因子-α、白细胞介素[IL]-1β、IL-6、IL-8 和 IL-10）的浓度。测试化合物均不影响调理或非调理的大肠杆菌的吞噬作用或氧化爆发活性。Pg-3-glc 和 PGA 在 0.08μmol/L 时增加了 IL-10 的浓度（分别为 P<.01 和 P<.001），但对肿瘤坏死因子-α、IL-1β、IL-6 和 IL-8 没有影响，其他化合物也没有影响。总之，本研究表明这些化合物对调理、吞噬和随后细菌的破坏没有影响。在生理相关浓度下，Pg-3-glc 和 PGA 具有抗炎特性；然而，作用温和，仅在测试的最低剂量下观察到，且仅限于 IL-10。
