海螺提取物对兽药残留控制分析中去氢甲睾酮测定的干扰。
Interference of Helix pomatia extracts on the determination of methandriol in veterinary residue control analysis.
作者信息
Leyssens L, Van Puymbroeck M, Raus J
机构信息
Dr. L. Willems-Instituut, Department of Residue Analysis, Diepenbeek, Belgium.
出版信息
Analyst. 1998 Dec;123(12):2643-4. doi: 10.1039/a805018j.
DOI:10.1039/a805018j
PMID:10435316
Abstract
Aqueous solutions containing methandriol (MAD) were incubated with Succus helix pomatia (SHP) or beta-glucuronidase from Escherichia coli (EC). SHP, used for enzymatic hydrolysis of urinary steroid conjugates for residue analysis of anabolic agents, caused transformation of MAD into methyltestosterone. No conversion occurred when bacterial beta-glucuronidase from E. coli was used.
摘要
将含有美雄醇（MAD）的水溶液与海螺酶（SHP）或来自大肠杆菌（EC）的β-葡萄糖醛酸酶一起孵育。用于对尿甾体结合物进行酶促水解以分析同化剂残留的SHP会导致MAD转化为甲基睾酮。使用来自大肠杆菌的细菌β-葡萄糖醛酸酶时未发生转化。
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