Lin L, Zhang H Y, Gu H M, Tang G Z, Zhang Z X, Wang J X, Zhang W N, Chen R S
School of Medicine, Nanjing University, China.
Zhongguo Yao Li Xue Bao. 1996 May;17(3):221-4.
To determine if nerve growth factors (NGF) can protect against glutamate-induced cortical neuron damage.
Neuron viability and lactate dehydrogenase (LDH) efflux in the bathing medium in primary cultures from 17-d-old mouse fetal cortex were measured to assay NGF effect. Imaging of the calcium indicator dye Fura-2 was used to measure the [Ca2+]i.
The LD50 for NGF-free glutamate was 0.2 mmol.L-1 (95% confidence limits 0-1.6 mmol.L-1). In the presence of NGF 60 micrograms.L-1, 59% of the neurons survived in glutamate 1.6 mmol.L-1. The protective effect afforded by NGF was maximal at 60 micrograms.L-1, at which it prevented the elevation in [Ca2+]i.
NGF protect cortical neurons against glutamate-induced toxicity via "stabilizing" [Ca2+]i level or suppression of the rise in [Ca2+]i.
确定神经生长因子（NGF）是否能保护皮质神经元免受谷氨酸诱导的损伤。
通过检测来自17日龄小鼠胚胎皮质的原代培养物中神经元的活力以及培养基中乳酸脱氢酶（LDH）的外流量来分析NGF的作用。使用钙指示剂Fura-2成像来测量细胞内钙离子浓度（[Ca2+]i）。
无NGF时谷氨酸的半数致死剂量（LD50）为0.2 mmol.L-1（95%置信区间0 - 1.6 mmol.L-1）。在存在60微克.L-1 NGF的情况下，1.6 mmol.L-1谷氨酸中59%的神经元存活。NGF在60微克.L-1时保护作用最大，此时它可防止[Ca2+]i升高。
NGF通过“稳定”[Ca2+]i水平或抑制[Ca2+]i升高来保护皮质神经元免受谷氨酸诱导的毒性作用。
