Liu W, Song Z J, Liang N C
Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang, China.
Zhongguo Yao Li Xue Bao. 1998 Nov;19(6):540-2.
To study the effects of genistein on aggregation and cytosolic free calcium concentration in platelets.
Using turbidimetry to analyse aggregation and using Fura-2 fluorescence technique to determine Ca2+ level.
Genistein strongly inhibited the pig platelet aggregation induced by thrombin (250 U.L-1). When genistein concentrations were 5 and 20 mumol.L-1, the inhibition rates on the aggregation were 52% and 73%, respectively. Genistein inhibited the rise of cytosolic free calcium concentration in platelets stimulated by thrombin (500 U.L-1) in the presence of extracellular Ca2+ 1 mmol.L-1. When genistein concentrations were 10, 20, 40, and 80 mumol.L-1, the inhibition rates were 24%, 40%, 63%, and 65%, respectively, but no effect on thrombin-induced internal Ca2+ release from dense tubular system.
Genistein is a potential anti-platelet agent, mainly due to an inhibition of Ca2+ influx.
