槲皮素对血小板聚集和细胞内游离钙的影响。
Effects of quercetin on aggregation and intracellular free calcium of platelets.
作者信息
Xiao D, Gu Z L, Bai J P, Wang Z
机构信息
Department of Pharmacology, Suzhou Medical College, China.
出版信息
Zhongguo Yao Li Xue Bao. 1995 May;16(3):223-6.
AIM
To study the effects of Que on the intraplatelet free calcium concentration and the effects of calcium on the inhibition of platelet aggregation by Que.
METHODS
Using Quin-2 fluorescence technique.
RESULTS
Que inhibited the platelet aggregation and the rise of [Ca2+]i induced by thrombin in platelets. The values of IC50 and 95% confidence interval were 146.2 (92.4 - 231.3) and 78.5 (49.5 - 124.4) mumol.L-1, respectively. The inhibitory effects of Que on platelet aggregation induced by thrombin were reduced by adding calcium to the medium, and Que had no effect on thrombin-induced internal Ca2+ release from dense tubular system.
CONCLUSION
The inhibitory effects of Que on aggregation and the rise of [Ca2+]i in platelets was mainly due to an inhibition of Ca2+ influx.
目的
研究槲皮素对血小板内游离钙浓度的影响以及钙对槲皮素抑制血小板聚集作用的影响。
方法
采用喹啉-2荧光技术。
结果
槲皮素抑制血小板聚集以及凝血酶诱导的血小板内[Ca2+]i升高。IC50值及95%置信区间分别为146.2（92.4 - 231.3）和78.5（49.5 - 124.4）μmol·L-1。向培养基中添加钙可降低槲皮素对凝血酶诱导的血小板聚集的抑制作用，且槲皮素对凝血酶诱导的致密管状系统内Ca2+释放无影响。
结论
槲皮素对血小板聚集及[Ca2+]i升高的抑制作用主要是由于抑制了Ca2+内流。
Zotero 插件