Huang C, Qin Y M, Liang N C
Department of Medical Biochemistry, Guangdong Medical College, Zhanjiang, China.
Zhongguo Yao Li Xue Bao. 1993 Nov;14(6):565-7.
Aprotinin inhibited platelet aggregation induced by thrombin (0.25 U.ml-1) with IC50 200 kIU.ml-1, and inhibited the rise of cytosolic free calcium concentration in platelets stimulated by thrombin (0.1 U.ml-1) in the absence and in the presence of Ca2+ 0.5 mmol.L-1 (IC50 117 and 50 kIU.ml-1, respectively), but had no effect on the amounts of actin and myosin heavy chain associated with cytoskeletons. These suggest that aprotinin is an anti-platelet agent and may exert its action through inhibiting the Ca2+ flux.
抑肽酶抑制凝血酶（0.25 U.ml-1）诱导的血小板聚集，IC50为200 kIU.ml-1，并且在不存在和存在0.5 mmol.L-1 Ca2+的情况下，抑制凝血酶（0.1 U.ml-1）刺激的血小板胞质游离钙浓度升高（IC50分别为117和50 kIU.ml-1），但对与细胞骨架相关的肌动蛋白和肌球蛋白重链的量没有影响。这些表明抑肽酶是一种抗血小板剂，可能通过抑制Ca2+通量发挥其作用。
