[多塞平对兔离体基底动脉环和隐动脉环的作用]

[Effects of doxepin on isolated basilar and saphenous artery rings of rabbits].

作者信息

Qi M, Hu X J, Lü A G, Ke J, Zhang G Q

机构信息

Department of Pharmacology, He-nan Medical University, Zhengzhou, China.

出版信息

Zhongguo Yao Li Xue Bao. 1998 Nov;19(6):572-4.

PMID:10437150

Abstract

AIM

To study the effects of doxepin (Dox) on cerebral artery.

METHODS

The effects of Dox were observed using the isolated basilar and saphenous artery rings of rabbits.

RESULTS

Dox inhibited the constriction of the basilar and saphenous artery rings evoked by KCl with IC50 5.75 mumol.L-1 (95% confidence limits were 2.3-14 mumol.L-1, n = 8) and 34.6 mumol.L-1 (95% confidence limits were 3.8-316 mumol.L-1, n = 8), respectively. Dox also inhibited the constriction of the basilar and saphenous artery rings of the rabbits stimulated by 5-hydroxytryptamine (5-HT), IC50 were 6.3 mumol.L-1 (95% confidence limits were 1.7-23.3 mumol.L-1, n = 7) and 8.0 mumol.L-1 (95% confidence limits were 6.3-10.3 mumol.L-1, n = 6), respectively. In both samples (basilar and saphenous artery rings) CaCl2 evoked, the pD2 of Dox was 5.28 +/- 0.40 and 4.76 +/- 0.14, respectively (n = 6, P < 0.01). Dox 5.8 mumol.L-1 inhibited the constriction of the saphenous artery evoked by norepinephrine (NE) in Ca(2+)-free medium. Dox 30 mumol.L-1 inhibited the constriction of the saphenous artery evoked both by NE and by readmission of CaCl2 (1.25 mmol.L-1).

CONCLUSION

As compared with its effect on the saphenous artery, Dox selectively inhibited the basilar artery.

摘要

目的

研究多塞平（Dox）对脑动脉的作用。

方法

采用兔离体基底动脉和隐动脉环观察Dox的作用。

结果

Dox抑制KCl诱发的基底动脉和隐动脉环收缩，其IC50分别为5.75 μmol·L-1（95%置信限为2.3 - 14 μmol·L-1，n = 8）和34.6 μmol·L-1（95%置信限为3.8 - 316 μmol·L-1，n = 8）。Dox还抑制5-羟色胺（5-HT）刺激兔的基底动脉和隐动脉环收缩，IC50分别为6.3 μmol·L-1（95%置信限为1.7 - 23.3 μmol·L-1，n = 7）和8.0 μmol·L-1（95%置信限为6.3 - 10.3 μmol·L-1，n = 6）。在两种标本（基底动脉和隐动脉环）中，CaCl2诱发收缩时，Dox的pD2分别为5.28 ± 0.40和4.76 ± 0.14（n = 6，P < 0.01）。5.8 μmol·L-1的Dox抑制无钙培养基中去甲肾上腺素（NE）诱发的隐动脉收缩。30 μmol·L-1的Dox抑制NE和再加入CaCl2（1.25 mmol·L-1）诱发的隐动脉收缩。