Borzi R M, Mazzetti I, Macor S, Silvestri T, Bassi A, Cattini L, Facchini A
Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, I.O.R., Bologna, Italy.
FEBS Lett. 1999 Jul 23;455(3):238-42. doi: 10.1016/s0014-5793(99)00886-8.
Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROalpha, MCP-1, RANTES, MIP-1alpha and MIP-1beta expression was evaluated by flow cytometric analysis and RT-PCR in chondrocytes isolated from cartilage specimens obtained from patients with osteoarthritis and rheumatoid arthritis and multiorgan donors as normal controls. All the chemokines except RANTES were found in normal chondrocytes, with different degrees of staining intensity. In osteoarthritis and rheumatoid arthritis patients, an enhancement of IL-8, GROalpha, MIP-1alpha and MIP-1beta was observed.
趋化因子在调节炎症部位的白细胞功能中起关键作用。为评估软骨细胞在炎症关节趋化环境中的作用，对CC和CXC趋化因子的细胞内含量进行了研究。通过流式细胞术分析和逆转录聚合酶链反应（RT-PCR），对从骨关节炎和类风湿关节炎患者以及作为正常对照的多器官供体的软骨标本中分离出的软骨细胞中的白细胞介素-8（IL-8）、α粒细胞趋化蛋白（GROα）、单核细胞趋化蛋白-1（MCP-1）、调节激活正常T细胞表达和分泌因子（RANTES）、巨噬细胞炎性蛋白-1α（MIP-1α）和巨噬细胞炎性蛋白-1β（MIP-1β）的表达进行了评估。除RANTES外，所有趋化因子在正常软骨细胞中均有发现，染色强度不同。在骨关节炎和类风湿关节炎患者中，观察到IL-8、GROα、MIP-1α和MIP-1β有所增强。
