Campo P, Loquet G, Blachère V, Roure M
Institut National de Recherche et de Sécurité, Laboratoire Multinuisances, Vandoeuvre, France.
Neurotoxicol Teratol. 1999 Jul-Aug;21(4):427-34. doi: 10.1016/s0892-0362(99)00010-0.
It is well established that organic solvents such as toluene and styrene are ototoxic in the rat; however, the intoxication route used to reach the organ of Corti is still questionable. The distribution of toluene and styrene in various tissues of Long-Evans rats (n = 2 x 8) was studied after inhalation of either 1750 ppm toluene or 1750 ppm styrene for 10 h (6 consecutive h + 4 h the following day). At the end of the solvent exposures, blood, brain, auditory nerves, the organ of Corti, cerebrospinal (CSF), and inner ear fluids (IEF) were sampled or removed to measure the rates of solvent uptake in each tissue by gas chromatography. Results indicate that CSF and IEF were free from detectable solvents, whereas the organ of Corti, the nerves, and the brain were contaminated. Therefore, both toluene- and styrene-induced hearing losses are caused by tissue intoxication rather than by fluid contamination. It is proposed that the outer sulcus is used as an intoxication route to reach the organ of Corti.
众所周知，甲苯和苯乙烯等有机溶剂对大鼠具有耳毒性；然而，用于到达柯蒂氏器的中毒途径仍存在疑问。在Long-Evans大鼠（n = 2×8）吸入1750 ppm甲苯或1750 ppm苯乙烯10小时（连续6小时 + 次日4小时）后，研究了甲苯和苯乙烯在其各种组织中的分布情况。在溶剂暴露结束时，采集或取出血液、大脑、听神经、柯蒂氏器、脑脊液（CSF）和内耳液（IEF），通过气相色谱法测量各组织中溶剂的摄取率。结果表明，脑脊液和内耳液中未检测到溶剂，而柯蒂氏器、神经和大脑受到了污染。因此，甲苯和苯乙烯引起的听力损失是由组织中毒而非液体污染所致。有人提出，外沟被用作到达柯蒂氏器的中毒途径。
