牛微卫星标记在非洲水牛（非洲野水牛）群体遗传学研究中的适用性

Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer).

作者信息

van Hooft W F, Hanotte O, Wenink P W, Groen A F, Sugimoto Y, Prins H H, Teale A

机构信息

Wageningen Agricultural University, Department of Environmental Sciences, Wageningen, The Netherlands.

出版信息

Anim Genet. 1999 Jun;30(3):214-20. doi: 10.1046/j.1365-2052.1999.00453.x.

DOI:10.1046/j.1365-2052.1999.00453.x

PMID:10442986

Abstract

The applicability of bovine autosomal microsatellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel of seven African buffalo. Amplification was observed for 139 markers (83%), and 101 markers were studied further with 91 (90%) being polymorphic. The mean number of alleles per marker was 5.0 (SE = 0.2) and the mean heterozygosity per marker was 0.61 (SE = 0.03). Considering the overall high level of polymorphism, it was concluded that most bovine microsatellite markers are applicable in African buffalo.

摘要

研究了牛常染色体微卫星标记在非洲水牛群体遗传学研究中的适用性。在由7头非洲水牛组成的测试组上，对总共168个微卫星标记进行了PCR扩增测试。观察到139个标记（83%）能够扩增，对其中101个标记进行了进一步研究，发现91个（90%）具有多态性。每个标记的平均等位基因数为5.0（标准误=0.2），每个标记的平均杂合度为0.61（标准误=0.03）。鉴于总体多态性水平较高，得出结论：大多数牛微卫星标记适用于非洲水牛。