Wood S, Nattress B, Kirkham J, Shore R, Brookes S, Griffiths J, Robinson C
Division of Oral Biology, Leeds Dental Institute, University of Leeds, UK.
J Photochem Photobiol B. 1999 May;50(1):1-7. doi: 10.1016/S1011-1344(99)00056-1.
Seven-day oral plaque biofilms have been formed on natural enamel surfaces in vivo using a previously reported in situ device. The devices are then incubated with a cationic Zn(II) phthalocyanine photosensitizer and irradiated with white light. Confocal scanning laser microscopy (CSLM) of the biofilms shows that the photosensitizer is taken up into the biomass of the biofilm and that significant cell death is caused by photodynamic therapy (PDT). In addition, the treated biofilms are much thinner than the control samples and demonstrate a different structure from the control samples, with little evidence of channels and a less dense biomass. Transmission electron microscopy (TEM) of the in vivo-formed plaque biofilms reveals considerable damage to bacteria in the biofilm, vacuolation of the cytoplasm and membrane damage being clearly visible after PDT. These results clearly demonstrate the potential value of PDT in the management of oral biofilms.
使用先前报道的原位装置在体内天然牙釉质表面形成了七天的口腔菌斑生物膜。然后将这些装置与阳离子锌（II）酞菁光敏剂一起孵育并用白光照射。生物膜的共聚焦扫描激光显微镜（CSLM）显示，光敏剂被吸收到生物膜的生物质中，并且光动力疗法（PDT）导致了显著的细胞死亡。此外，经处理的生物膜比对照样品薄得多，并且显示出与对照样品不同的结构，几乎没有通道迹象且生物质密度较低。体内形成的菌斑生物膜的透射电子显微镜（TEM）显示，生物膜中的细菌受到相当大的损伤，PDT后细胞质空泡化和膜损伤清晰可见。这些结果清楚地证明了PDT在口腔生物膜管理中的潜在价值。
