Department of Dental Materials and Prosthodontics, Araraquara Dental School, UNESP-Universidade Estadual Paulista, SP, Brazil.
Photochem Photobiol. 2013 Jan-Feb;89(1):111-9. doi: 10.1111/j.1751-1097.2012.01198.x. Epub 2012 Aug 8.
New drug delivery systems, such as nanoemulsions (NE), have been developed to allow the use of hydrophobic drugs on the antimicrobial photodynamic therapy. This study evaluated the photodynamic potential of aluminum-chloride-phthalocyanine (ClAlPc) entrapped in cationic and anionic NE to inactivate Candida albicans planktonic cultures and biofilm compared with free ClAlPc. Fungal suspensions were treated with different delivery systems containing ClAlPc and light emitting diode. For planktonic suspensions, colonies were counted and cell metabolism was evaluated by XTT assay. Flow cytometry evaluated cell membrane damage. For biofilms, the metabolic activity was evaluated by XTT and ClAlPc distribution through biofilms was analyzed by confocal laser scanning microscopy (CLSM). Fungal viability was dependent on the delivery system, superficial charge and light dose. Free ClAlPc caused photokilling of the yeast when combined with 100 J cm(-2). Cationic NE-ClAlPc reduced significantly both colony counts and cell metabolism (P < 0.05). In addition, cationic NE-ClAlPc and free ClAlPc caused significant damage to the cell membrane (P < 0.05). For the biofilms, cationic NE-ClAlPc reduced cell metabolism by 70%. Anionic NE-ClAlPc did not present antifungal activity. CLSM showed different accumulation on biofilms between the delivery systems. Although NE system showed a lower activity for planktonic culture, cationic NE-ClAlPc showed better results for Candida biofilms.
新型药物递送系统，如纳米乳剂（NE），已经被开发出来，以使疏水性药物能够用于抗菌光动力疗法。本研究评估了包封在阳离子和阴离子 NE 中的铝-氯化-酞菁（ClAlPc）在失活白色念珠菌浮游培养物和生物膜方面的光动力潜力，并与游离 ClAlPc 进行了比较。真菌悬浮液用含有 ClAlPc 和发光二极管的不同递送系统处理。对于浮游悬浮液，通过平板计数法计算菌落并通过 XTT 测定法评估细胞代谢。流式细胞术评估细胞膜损伤。对于生物膜，通过 XTT 评估代谢活性，并通过共聚焦激光扫描显微镜（CLSM）分析 ClAlPc 在生物膜中的分布。真菌活力取决于递送系统、表面电荷和光剂量。游离 ClAlPc 与 100 J cm(-2) 结合时可导致酵母的光杀伤。阳离子 NE-ClAlPc 显著降低菌落计数和细胞代谢（P < 0.05）。此外，阳离子 NE-ClAlPc 和游离 ClAlPc 对细胞膜造成显著损伤（P < 0.05）。对于生物膜，阳离子 NE-ClAlPc 使细胞代谢降低了 70%。阴离子 NE-ClAlPc 没有表现出抗真菌活性。CLSM 显示不同的递送系统在生物膜上的积累情况不同。尽管 NE 系统对浮游培养物的活性较低，但阳离子 NE-ClAlPc 对白色念珠菌生物膜的效果更好。
