Stiedl O, Palve M, Radulovic J, Birkenfeld K, Spiess J
Department of Molecular Neuroendocrinology, Max Planck Institute for Experimental Medicine, Goettingen, Germany.
Behav Neurosci. 1999 Jun;113(3):496-506. doi: 10.1037//0735-7044.113.3.496.
A 1-trial fear conditioning was used to investigate the temporal development of fear responses expressed as increase of freezing or heart rate and its impairment by the protein synthesis inhibitor cycloheximide (CHX) in male C57BL/6N mice. Heart rate was measured with an implanted transmitter. In the memory tests, mice were exposed to tone and context provided either as foreground or background stimulus during training. The fear responses developed differently from 0 to 24 hr after training under these 3 conditions. A single pretraining CHX injection impaired both memory forms, whereas a single posttraining CHX injection impaired tone- but not context-dependent memory, with the context provided as background stimulus. It was concluded that consolidation of tone-, foreground context-, and background context-dependent fear conditioning may be mediated by partly different neuronal or partly different biochemical pathways, or both.
采用单次试验恐惧条件反射来研究雄性C57BL/6N小鼠中以僵立增加或心率增加表示的恐惧反应的时间发展，以及蛋白质合成抑制剂环己酰亚胺（CHX）对其的损害。心率通过植入式发射器进行测量。在记忆测试中，小鼠在训练期间暴露于作为前景或背景刺激提供的音调与环境中。在这三种条件下，训练后0至24小时恐惧反应的发展有所不同。单次训练前注射CHX会损害两种记忆形式，而单次训练后注射CHX会损害音调依赖性记忆，但不损害背景刺激提供的环境依赖性记忆。得出的结论是，音调依赖性、前景环境依赖性和背景环境依赖性恐惧条件反射的巩固可能由部分不同的神经元途径或部分不同的生化途径介导，或由两者共同介导。
