Hayes B B, Meade B J
National Institute for Occupational Safety and Health, Health Effects Laboratory Division, Morgantown, WV 26505, USA.
Drug Chem Toxicol. 1999 Aug;22(3):491-506. doi: 10.3109/01480549909042528.
Given the increasing prevalence of occupational sensitization to acrylate compounds, n-butyl acrylate (BAC), ethyl acrylate (EAC), and trimethylol propane triacrylate (TMT) were recommended by the National Toxicology Program for hypersensitivity testing in female B6C3F1 mice. The objectives of these studies were to determine the irritating and sensitizing potential of these three compounds using an irritation assay, the murine Local Lymph Node Assay (LLNA), and the Mouse Ear Swelling Test (MEST). The minimal irritating concentration for TMT was determined to be 1.0%, whereas BAC and EAC demonstrated no irritation up to 30%, the highest concentration tested. TMT tested positive in the LLNA at concentrations as low as 0.1% whereas an induction concentration of 0.3% was required to elicit a positive response in the MEST. Furthermore, BAC tested negative in the MEST at induction concentrations as high as 30%, but yielded positive results in the LLNA at concentrations as low as 20%. EAC, at all concentrations tested, was negative in both the MEST and the LLNA. Cross reactivity was only seen when mice were sensitized with TMT and challenged with BAC. In these studies, the LLNA was a more sensitive indicator of the allergic potential of these three acrylates when compared to the MEST.
鉴于职业性对丙烯酸酯化合物致敏的患病率不断上升，美国国家毒理学计划推荐对雌性B6C3F1小鼠进行丙烯酸正丁酯（BAC）、丙烯酸乙酯（EAC）和三羟甲基丙烷三丙烯酸酯（TMT）的超敏反应测试。这些研究的目的是使用刺激试验、小鼠局部淋巴结试验（LLNA）和小鼠耳肿胀试验（MEST）来确定这三种化合物的刺激和致敏潜力。TMT的最低刺激浓度被确定为1.0%，而BAC和EAC在高达30%（测试的最高浓度）时未显示出刺激作用。TMT在低至0.1%的浓度下在LLNA中呈阳性，而在MEST中需要0.3%的诱导浓度才能引发阳性反应。此外，BAC在高达30%的诱导浓度下在MEST中呈阴性，但在低至20%的浓度下在LLNA中产生阳性结果。EAC在所有测试浓度下，在MEST和LLNA中均为阴性。只有当小鼠用TMT致敏并用BAC攻击时才出现交叉反应。在这些研究中，与MEST相比，LLNA是这三种丙烯酸酯过敏潜力更敏感的指标。
