Fillet M, Bechet I, Piette V, Crommen J
Department of Analytical, Pharmaceutical Chemistry, Institute of Pharmacy, University of Liège, Belgium.
Electrophoresis. 1999 Jul;20(9):1907-15. doi: 10.1002/(SICI)1522-2683(19990701)20:9<1907::AID-ELPS1907>3.0.CO;2-L.
The aim of the present work was to investigate the separation of nonsteroidal anti-inflammatory drugs (NSAIDs: niflumic acid, flufenamic acid, piroxicam, alclofenac, tiaprofenic acid, flurbiprofen, suprofen, ketoprofen, naproxen, indomethacin, carprofen, indoprofen, sulindac) in capillary electrophoresis (CE) using completely nonaqueous systems. The influence of different parameters such as nature and proportion of organic solvent (methanol, acetonitrile, 2-propanol), apparent pH (ranging from 7 to 9) and temperature (ranging from 25 to 40 degrees C) on selectivity and migration times were studied systematically in an uncoated fused-silica capillary. A nonaqueous electrolyte made of 50 mM ammonium acetate - 13.75 mM ammonia in methanol proved to resolve 11 NSAIDs at 25 degrees C and 13 NSAIDs at 36 degrees C, both within 13 min and without a modifier besides the methanol itself. The same buffer containing 30% acetonitrile provides a satisfactory separation for 13 NSAIDs within 14 min at 25 degrees C.
本研究的目的是利用完全非水体系研究毛细管电泳（CE）中分离非甾体抗炎药（NSAIDs：尼氟酸、氟芬那酸、吡罗昔康、阿氯芬酸、噻洛芬酸、氟比洛芬、舒洛芬、酮洛芬、萘普生、吲哚美辛、卡洛芬、吲哚洛芬、舒林酸）的方法。在未涂层熔融石英毛细管中，系统研究了不同参数如有机溶剂（甲醇、乙腈、异丙醇）的性质和比例、表观pH值（7至9）以及温度（25至40℃）对选择性和迁移时间的影响。由50 mM醋酸铵 - 13.75 mM氨在甲醇中制成的非水电解质在25℃时可分离11种NSAIDs，在36℃时可分离13种NSAIDs，均在13分钟内完成，且除甲醇本身外无需改性剂。含有30%乙腈的相同缓冲液在25℃下14分钟内可对13种NSAIDs提供令人满意的分离效果。
