Qi Shengda, Ding Lan, Tian Kan, Chen Xingguo, Hu Zhide
Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
J Pharm Biomed Anal. 2006 Jan 23;40(1):35-41. doi: 10.1016/j.jpba.2005.06.003. Epub 2005 Jul 11.
Three bioactive triterpenes ursolic acid, oleanolic acid and 2alpha,3beta,24-trihydroxy-urs-12-en-28-oic acid were simultaneously separated by nonaqueous capillary electrophoresis (NACE) with methanol:acetonitrile (65:35 v/v) mixture containing 90 mm trishydroxymethylaminomethane (Tris) at an applied voltage of +25 kV and a hydrodynamic injection of 5s. The effect of solvent composition, electrolyte nature and concentration on the electrophoretic behavior of the analytes were systematically studied. Separations were carried out in a fused-silica capillary tube with UV detection at 214 nm. Good separation and correlation coefficients were obtained. Meanwhile, the method was applied to separation and determination the three components in six Chinese herbs extraction. It is concluded that this method could be used for speedy and accurate qualitative and quantitative analysis of bioactive triterpenes in Chinese herbs.
采用非水毛细管电泳（NACE）法，以甲醇：乙腈（65:35 v/v）混合液为溶剂，其中含有90 mM三羟甲基氨基甲烷（Tris），在+25 kV的外加电压下，通过5 s的流体动力学进样，同时分离了三种生物活性三萜类化合物：熊果酸、齐墩果酸和2α,3β,24-三羟基-urs-12-烯-28-酸。系统研究了溶剂组成、电解质性质和浓度对分析物电泳行为的影响。在熔融石英毛细管中进行分离，并在214 nm处进行紫外检测。获得了良好的分离效果和相关系数。同时，该方法应用于六种中草药提取物中三种成分的分离和测定。结果表明，该方法可用于中草药中生物活性三萜类化合物的快速、准确的定性和定量分析。
