Nakamura C V, Waldow L, Pelegrinello S R, Ueda-Nakamura T, Filho B A, Filho B P
Laboratório de Microbiologia, Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Estadual de Maringá, Maringá, PR, 87020-900, Brasil.
Mem Inst Oswaldo Cruz. 1999 Jul-Aug;94(4):519-25. doi: 10.1590/s0074-02761999000400017.
Fatty acid and sterol analysis were performed on Phytomonas serpens and Phytomonas sp. grown in chemically defined and complex medium, and P. françai cultivated in complex medium. The three species of the genus Phytomonas had qualitatively identical fatty acid patterns. Oleic, linoleic, and linolenic were the major unsaturated fatty acids. Miristic and stearic were the major saturated fatty acids. Ergosterol was the only sterol isolated from Phytmonas sp. and P. serpens grown in a sterol-free medium, indicating that it was synthesized de novo. When P. françai that does not grow in defined medium was cultivated in a complex medium, cholesterol was the only sterol detected. The fatty acids and sterol isolated from Phytomonas sp. and P. serpens grown in a chemically defined lipid-free medium indicated that they were able to biosynthesize fatty acids and ergosterol from acetate or from acetate precursors such as glucose or threonine.
对在化学成分明确的培养基和复合培养基中生长的蛇形植鞭毛虫（Phytomonas serpens）和植鞭毛虫属（Phytomonas sp.），以及在复合培养基中培养的弗朗氏植鞭毛虫（P. françai）进行了脂肪酸和固醇分析。植鞭毛虫属的这三个物种具有定性相同的脂肪酸模式。油酸、亚油酸和亚麻酸是主要的不饱和脂肪酸。肉豆蔻酸和硬脂酸是主要的饱和脂肪酸。麦角固醇是从在无固醇培养基中生长的植鞭毛虫属（Phytmonas sp.）和蛇形植鞭毛虫中分离出的唯一固醇，这表明它是从头合成的。当不在限定培养基中生长的弗朗氏植鞭毛虫在复合培养基中培养时，检测到的唯一固醇是胆固醇。从在化学成分明确的无脂培养基中生长的植鞭毛虫属（Phytomonas sp.）和蛇形植鞭毛虫中分离出的脂肪酸和固醇表明，它们能够从乙酸盐或乙酸盐前体（如葡萄糖或苏氨酸）生物合成脂肪酸和麦角固醇。
