Buttke T M, Jones S D, Bloch K
J Bacteriol. 1980 Oct;144(1):124-30. doi: 10.1128/jb.144.1.124-130.1980.
Saccharomyces cerevisiae GL7 cells require exogenous sterol and unsaturated fatty acid for growth. When grown in the presence of cholesterol or 7-dehydrocholesterol, the cells incorporated less saturated fatty acid into phospholipids than cells grown with ergosterol, stigmasterol, or beta-sitosterol as the sterol source. This lower saturated fatty acid content was most pronounced in phosphatidylethanolamine, slightly less so in phosphatidylcholine, and least evident in phosphatidylserine and phosphatidylinositol. Growing the cells with the various sterols did not affect the ratios of individual phospholipids. The ability of strain GL7 to use 7-dehydrocholesterol as the only sterol supplement for growth was dependent upon the nature of the unsaturated fatty acids added to the growth medium. In the presence of linoleic, linolenic, or a mixture of palmitoleic and oleic acids, excellent growth was observed with either ergosterol, cholesterol, or 7-dehydrocholesterol. However, when the medium was supplemented with either oleic or petroselenic acid, the cells grew more slowly (oleic) or much more poorly (petroselenic) with 7-dehydrocholesterol than with ergosterol. A specific relationship between sterol structure and membrane fatty acid composition in yeast cells is implied.
酿酒酵母GL7细胞生长需要外源性固醇和不饱和脂肪酸。当在胆固醇或7-脱氢胆固醇存在的情况下生长时，与以麦角固醇、豆甾醇或β-谷甾醇作为固醇来源生长的细胞相比，这些细胞掺入磷脂中的饱和脂肪酸更少。这种较低的饱和脂肪酸含量在磷脂酰乙醇胺中最为明显，在磷脂酰胆碱中稍少，而在磷脂酰丝氨酸和磷脂酰肌醇中最不明显。用各种固醇培养细胞不会影响单个磷脂的比例。GL7菌株将7-脱氢胆固醇用作唯一的固醇补充物来生长的能力取决于添加到生长培养基中的不饱和脂肪酸的性质。在亚油酸、亚麻酸或棕榈油酸和油酸的混合物存在的情况下，用麦角固醇、胆固醇或7-脱氢胆固醇都能观察到良好的生长。然而，当培养基中添加油酸或岩芹酸时，与用麦角固醇相比，细胞用7-脱氢胆固醇生长得更慢（油酸）或更差得多（岩芹酸）。这暗示了酵母细胞中固醇结构与膜脂肪酸组成之间存在特定关系。
