Adagu I S, Warhurst D C
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK.
Parasitology. 1999 Jul;119 ( Pt 1):1-6. doi: 10.1017/s0031182099004382.
An allele-specific, one tube PCR for the sensitive and reliable detection of point mutations in Plasmodium falciparum DNA is described. Design of specific internal primers and optimization of the PCR is simple, and the procedure is robust and sensitive. Single nucleotide polymorphisms at codons 184, 1034, 1042 and 1246 of the P, falciparum multidrug resistance gene Pfmdr1, were examined in 6 laboratory isolates, to validate the technique.
本文描述了一种等位基因特异性单管PCR方法，用于灵敏且可靠地检测恶性疟原虫DNA中的点突变。特异性内部引物的设计和PCR的优化很简单，该方法稳健且灵敏。为验证该技术，我们检测了6个实验室分离株中恶性疟原虫多药耐药基因Pfmdr1第184、1034、1042和1246密码子处的单核苷酸多态性。
