与牛白血病病毒感染相关的两种分离抗原的特性。

Properties of two isolated antigens associated with bovine leukemia virus infection.

作者信息

Onuma M, Olson C, Driscoll D M

出版信息

J Natl Cancer Inst. 1976 Sep;57(3):571-8. doi: 10.1093/jnci/57.3.571.

DOI:10.1093/jnci/57.3.571

Abstract

We isolated an ether-resistant internal antigen and an ether-sensitive antigen previously described in relation to bovine leukemia virus infection. These two antigens have now been isolated by isoelectric focusing and concanavalin A affinity chromatography, respectively. The ether-resistant antigen exhibited isoelectric heterogeneity with a major peak at pH 7.2 and a minor peak at pH 6.2. Its molecular weight, estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was 23,000 (p23), and it gave a sedimentation value of 2.3s. For material containing ether-sensitive antigen, analyzed by SDS-PAGE, protein staining revealed four components with molecular weights of 18,000, 25,000, 45,000, and 55,000. Two of these [45,000 (gp45) and 55,000 (gp55)] were stained by periodic acid-Schiff reagent. Isoelectric point and sedimentation value of the major glycoprotein (gp45) were pH 5.0 and 3.4s, respectively; no immunologic cross-reactivity was found between p23 and glycoprotein antigen.

摘要

我们分离出一种抗乙醚内部抗原和一种先前已报道的与牛白血病病毒感染相关的乙醚敏感抗原。现在，这两种抗原已分别通过等电聚焦和伴刀豆球蛋白A亲和层析法分离出来。抗乙醚抗原表现出等电不均一性，在pH 7.2处有一个主峰，在pH 6.2处有一个次峰。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）估计其分子量为23,000（p23），沉降值为2.3s。对于含有乙醚敏感抗原的材料，经SDS-PAGE分析，蛋白质染色显示有四种分子量分别为18,000、25,000、45,000和55,000的成分。其中两种[45,000（gp45）和55,000（gp55）]被过碘酸-希夫试剂染色。主要糖蛋白（gp45）的等电点和沉降值分别为pH 5.0和3.4s；在p23和糖蛋白抗原之间未发现免疫交叉反应。