Kumar M, Behera A K, Matsuse H, Lockey R F, Mohapatra S S
Joy McCann Culverhouse, Airway Disease Research Center, Division of Allergy and Immunology, Department of Internal Medicine, VA Hospital, Tampa, FL 33612, USA.
Immunology. 1999 Jul;97(3):515-21. doi: 10.1046/j.1365-2567.1999.00782.x.
The tubercle vaccine, bacille Calmette-Guérin (BCG), is a strong inducer of T-helper type 1 (Th1) responsiveness, and it has been suggested that recombinant BCG (rBCG), which produces and secretes antigens, may be used to prevent allergic diseases. The effects of rBCG vaccination on allergic responses in a murine model were examined in this study. A BCG-Escherichia coli shuttle vector was developed with the promoter and signal sequence of the alpha-antigen of Mycobacterium bovis, and the vector was tested using E. coli beta-galactosidase as the model antigen and allergen. This vector enabled the expression of the E. coli beta-galactosidase gene in BCG, which was detected in its protein extract by immunoblotting analysis. Vaccination of mice with a single dose of 106 recombinant BCG generated a beta-galactosidase-specific antibody response. The splenocytes of vaccinated mice compared with controls produced significantly higher amounts of interferon-gamma (IFN-gamma) (P<0. 01) and interleukin-2 (IL-2) (P<0.05) and lower amounts of IL-5 (P<0. 01). Mice vaccinated with rBCG had significantly less (P<0.01) serum IgE compared with controls. These results together demonstrate that rBCG secreting antigens or allergens may be utilized for the induction of a Th1-like response and the down-regulation of IgE antibody response.
结核疫苗卡介苗（BCG）是1型辅助性T细胞（Th1）反应的强力诱导剂，有人提出，能够产生并分泌抗原的重组卡介苗（rBCG）可用于预防过敏性疾病。本研究检测了rBCG疫苗接种对小鼠模型过敏反应的影响。利用牛分枝杆菌α抗原的启动子和信号序列构建了一种卡介苗-大肠杆菌穿梭载体，并以大肠杆菌β-半乳糖苷酶作为模型抗原和变应原对该载体进行检测。该载体使大肠杆菌β-半乳糖苷酶基因能够在卡介苗中表达，通过免疫印迹分析在其蛋白提取物中检测到了该基因。用单剂量106的重组卡介苗对小鼠进行疫苗接种产生了β-半乳糖苷酶特异性抗体反应。与对照组相比，接种疫苗小鼠的脾细胞产生的γ干扰素（IFN-γ）（P<0.01）和白细胞介素-2（IL-2）（P<0.05）显著增多，而IL-5的产生量减少（P<0.01）。与对照组相比，接种rBCG的小鼠血清IgE显著减少（P<0.01）。这些结果共同表明，分泌抗原或变应原的rBCG可用于诱导Th1样反应并下调IgE抗体反应。
