一种用于重组分枝杆菌的表位递送系统。
An epitope delivery system for use with recombinant mycobacteria.
作者信息
Hetzel C, Janssen R, Ely S J, Kristensen N M, Bunting K, Cooper J B, Lamb J R, Young D B, Thole J E
机构信息
Department of Biology, Imperial College School of Medicine, London, United Kingdom.
出版信息
Infect Immun. 1998 Aug;66(8):3643-8. doi: 10.1128/IAI.66.8.3643-3648.1998.
Abstract
We have developed a novel epitope delivery system based on the insertion of peptides within a permissive loop of a bacterial superoxide dismutase molecule. This system allowed high-level expression of heterologous peptides in two mycobacterial vaccine strains, Mycobacterium bovis bacille Calmette-Guérin (BCG) and Mycobacterium vaccae. The broader application of the system was analyzed by preparation of constructs containing peptide epitopes from a range of infectious agents and allergens. We report detailed characterization of the immunogenicity of one such construct, in which an epitope from the Der p1 house dust mite allergen was expressed in M. vaccae. The construct was able to stimulate T-cell hybridomas specific for Der p1, and it induced peptide-specific gamma interferon responses when used to immunize naive mice. This novel expression system demonstrates new possibilities for the use of mycobacteria as vaccine delivery vehicles.
摘要
我们基于将肽插入细菌超氧化物歧化酶分子的一个允许性环内，开发了一种新型表位递送系统。该系统能使两种分枝杆菌疫苗菌株，即牛分枝杆菌卡介苗（BCG）和母牛分枝杆菌中异源肽实现高水平表达。通过制备包含来自一系列传染原和变应原的肽表位的构建体，分析了该系统更广泛的应用。我们报告了其中一种构建体免疫原性的详细特征，该构建体中来自屋尘螨变应原Der p1的一个表位在母牛分枝杆菌中表达。该构建体能够刺激对Der p1特异的T细胞杂交瘤，并且当用于免疫未接触过抗原的小鼠时，它能诱导肽特异性γ干扰素应答。这种新型表达系统展示了将分枝杆菌用作疫苗递送载体的新可能性。
相似文献
1
An epitope delivery system for use with recombinant mycobacteria.一种用于重组分枝杆菌的表位递送系统。
Infect Immun. 1998 Aug;66(8):3643-8. doi: 10.1128/IAI.66.8.3643-3648.1998.
2
Immunogenicity of recombinant Mycobacterium bovis bacille Calmette-Guèrin clones expressing T and B cell epitopes of Mycobacterium tuberculosis antigens.表达结核分枝杆菌抗原 T 和 B 细胞表位的重组牛分枝杆菌卡介苗克隆的免疫原性。
BMC Immunol. 2013;14 Suppl 1(Suppl 1):S5. doi: 10.1186/1471-2172-14-S1-S5. Epub 2013 Feb 25.
3
Analysis of recombinant mycobacteria as T helper type 1 vaccines in an allergy challenge model.在过敏激发模型中对作为1型辅助性T细胞疫苗的重组分枝杆菌进行分析。
Immunology. 2001 Apr;102(4):441-9. doi: 10.1046/j.1365-2567.2001.01207.x.
4
Accelerated induction of mycobacterial antigen-specific CD8+ T cells in the Mycobacterium tuberculosis-infected lung by subcutaneous vaccination with Mycobacterium bovis bacille Calmette-Guérin.经皮接种牛型结核分枝杆菌卡介苗加速结核分枝杆菌感染肺部中分枝杆菌抗原特异性 CD8+T 细胞的诱导。
Immunology. 2009 Dec;128(4):556-63. doi: 10.1111/j.1365-2567.2009.03141.x.
5
Immunogenicity and protective efficacy of recombinant Bacille Calmette-Guerin strains expressing mycobacterium antigens Ag85A, CFP10, ESAT-6, GM-CSF and IL-12p70.表达分枝杆菌抗原Ag85A、CFP10、ESAT-6、GM-CSF和IL-12p70的重组卡介苗菌株的免疫原性和保护效力
Hum Vaccin Immunother. 2017 Jun 3;13(6):1-8. doi: 10.1080/21645515.2017.1279771. Epub 2017 Feb 14.
6
Dendritic cells infected with Mycobacterium bovis bacillus Calmette Guerin activate CD8(+) T cells with specificity for a novel mycobacterial epitope.感染卡介苗的树突状细胞可激活对一种新型分枝杆菌表位具有特异性的CD8(+) T细胞。
Int Immunol. 2001 Apr;13(4):451-8. doi: 10.1093/intimm/13.4.451.
7
Dendritic cells induce CD4+ and CD8+ T-cell responses to Mycobacterium bovis and M. avium antigens in Bacille Calmette Guérin vaccinated and nonvaccinated cattle.树突状细胞在卡介苗接种和未接种的牛中诱导针对牛分枝杆菌和鸟分枝杆菌抗原的CD4+和CD8+ T细胞反应。
Scand J Immunol. 2000 Sep;52(3):285-91. doi: 10.1046/j.1365-3083.2000.00780.x.
8
Recombinant BCG coexpressing Ag85B, ESAT-6 and Rv3620c elicits specific Th1 immune responses in C57BL/6 mice.共表达Ag85B、ESAT-6和Rv3620c的重组卡介苗在C57BL/6小鼠中引发特异性Th1免疫反应。
Microb Pathog. 2014 Apr-May;69-70:53-9. doi: 10.1016/j.micpath.2014.03.011. Epub 2014 Apr 12.
9
Immunogenicity and protective efficacy of mycobacterial DNA vaccines incorporating plasmid-encoded cytokines against Mycobacterium bovis.含质粒编码细胞因子的分枝杆菌 DNA 疫苗对牛分枝杆菌的免疫原性和保护效力。
Immunol Cell Biol. 2010 Aug;88(6):651-7. doi: 10.1038/icb.2010.25. Epub 2010 Mar 16.
10
Improved immunogenicity of recombinant Mycobacterium bovis bacillus Calmette-Guérin strains expressing fusion protein Ag85A-ESAT-6 of Mycobacterium tuberculosis.表达结核分枝杆菌融合蛋白 Ag85A-ESAT-6 的重组牛型分枝杆菌卡介苗菌株的免疫原性增强。
Scand J Immunol. 2010 Oct;72(4):332-8. doi: 10.1111/j.1365-3083.2010.02444.x.
引用本文的文献
1
CD4 T cell memory is impaired by species-specific cytotoxic differentiation, but not by TCF-1 loss.CD4 T 细胞记忆受到种属特异性细胞毒性分化的损害，但不受 TCF-1 缺失的影响。
Front Immunol. 2023 Apr 14;14:1168125. doi: 10.3389/fimmu.2023.1168125. eCollection 2023.
2
Generation of CD8+ T-cell responses by a recombinant nonpathogenic Mycobacterium smegmatis vaccine vector expressing human immunodeficiency virus type 1 Env.由表达1型人类免疫缺陷病毒Env的重组非致病性耻垢分枝杆菌疫苗载体产生CD8 + T细胞应答。
J Virol. 2006 Feb;80(4):1645-52. doi: 10.1128/JVI.80.4.1645-1652.2006.
3
Analysis of recombinant mycobacteria as T helper type 1 vaccines in an allergy challenge model.在过敏激发模型中对作为1型辅助性T细胞疫苗的重组分枝杆菌进行分析。
Immunology. 2001 Apr;102(4):441-9. doi: 10.1046/j.1365-2567.2001.01207.x.
4
Identification of promiscuous epitopes from the Mycobacterial 65-kilodalton heat shock protein recognized by human CD4(+) T cells of the Mycobacterium leprae memory repertoire.从麻风分枝杆菌记忆库中人类CD4(+) T细胞识别的分枝杆菌65千道尔顿热休克蛋白中鉴定混杂表位。
Infect Immun. 1999 Nov;67(11):5683-9. doi: 10.1128/IAI.67.11.5683-5689.1999.
5
A recombinant BCG vaccine generates a Th1-like response and inhibits IgE synthesis in BALB/c mice.一种重组卡介苗疫苗可在BALB/c小鼠中产生类似Th1的反应并抑制IgE合成。
Immunology. 1999 Jul;97(3):515-21. doi: 10.1046/j.1365-2567.1999.00782.x.
本文引用的文献
1
Prediction of murine MHC class I epitopes in a major house dust mite allergen and induction of T1-type CD8+ T cell responses.主要屋尘螨变应原中鼠类MHC I类表位的预测及T1型CD8 + T细胞应答的诱导
Int Immunol. 1997 Feb;9(2):273-80. doi: 10.1093/intimm/9.2.273.
2
Major histocompatibility class I presentation of soluble antigen facilitated by Mycobacterium tuberculosis infection.结核分枝杆菌感染促进可溶性抗原的主要组织相容性复合体I类呈递。
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11786-91. doi: 10.1073/pnas.93.21.11786.
3
Induction of T cell responses to the invariant chain derived peptide CLIP in mice immunized with the group 1 allergen of house dust mite.在用屋尘螨1组变应原免疫的小鼠中诱导T细胞对恒定链衍生肽CLIP的反应。
Int Immunol. 1996 Jul;8(7):1091-8. doi: 10.1093/intimm/8.7.1091.
4
Molecular characterization of a surface-exposed superoxide dismutase of Mycobacterium avium.鸟分枝杆菌表面暴露超氧化物歧化酶的分子特征
Microb Pathog. 1996 Jan;20(1):41-55. doi: 10.1006/mpat.1996.0004.
5
Adjuvants for human vaccines--current status, problems and future prospects.人类疫苗佐剂——现状、问题与未来前景
Vaccine. 1995 Oct;13(14):1263-76. doi: 10.1016/0264-410x(95)00011-o.
6
Comparison of antigen presentation by lymph node cells from protein and peptide-primed mice.蛋白质致敏小鼠和肽致敏小鼠的淋巴结细胞抗原呈递比较。
Immunology. 1993 Jan;78(1):58-64.
7
Sequence polymorphisms of cDNA clones encoding the mite allergen Der p I.
Int Arch Allergy Immunol. 1993;101(4):364-8. doi: 10.1159/000236478.
8
Protective immunity elicited by recombinant bacille Calmette-Guerin (BCG) expressing outer surface protein A (OspA) lipoprotein: a candidate Lyme disease vaccine.表达外表面蛋白A（OspA）脂蛋白的重组卡介苗（BCG）引发的保护性免疫：一种莱姆病候选疫苗。
J Exp Med. 1993 Jul 1;178(1):197-209. doi: 10.1084/jem.178.1.197.
9
Crystallization and preliminary X-ray analysis of the superoxide dismutase from Mycobacterium tuberculosis.结核分枝杆菌超氧化物歧化酶的结晶及初步X射线分析
J Mol Biol. 1994 Jan 21;235(3):1156-8. doi: 10.1006/jmbi.1994.1068.
10
Transformation of mycobacterial species using hygromycin resistance as selectable marker.以潮霉素抗性作为选择标记对分枝杆菌属进行转化。
Microbiology (Reading). 1994 Jan;140 ( Pt 1):133-8. doi: 10.1099/13500872-140-1-133.
Zotero 插件