Maldonado-López R, De Smedt T, Pajak B, Heirman C, Thielemans K, Leo O, Urbain J, Maliszewski C R, Moser M
Département de Biologie Moléculaire, Université Libre de Bruxelles, Rhode-Saint-Genèse, Belgium.
J Leukoc Biol. 1999 Aug;66(2):242-6. doi: 10.1002/jlb.66.2.242.
Data from adoptive transfer of mature dendritic cells (DC) indicate that they are responsible for the induction of primary immunity. Two subclasses of DC have been recently identified in spleen that differ in their phenotype and in certain regulatory features. In vitro, both subsets have the capacity to activate naive T cells, although CD8a+ DC have been shown to induce T cell apoptosis and to stimulate lower levels of cytokines compared with CD8alpha- DC. The objective of this study was to analyze the function of these distinct DC types in vivo. Our results show that both subsets, pulsed extracorporeally with antigen and injected in the footpads of syngeneic mice, sensitize an antigen-specific T cell primary response. However, CD8alpha+ cells trigger the development of Thl-type cells, whereas CD8alpha- DC induce a Th2-type response. These observations suggest that the Th1/Th2 balance in vivo is regulated by the antigen-presenting-cells of the primary immune responses.
成熟树突状细胞（DC）过继转移的数据表明，它们负责诱导初始免疫。最近在脾脏中鉴定出了DC的两个亚类，它们在表型和某些调节特征上有所不同。在体外，尽管与CD8α- DC相比，CD8a + DC已显示出诱导T细胞凋亡并刺激较低水平的细胞因子，但这两个亚群都具有激活初始T细胞的能力。本研究的目的是分析这些不同类型的DC在体内的功能。我们的结果表明，这两个亚群在体外与抗原脉冲后注射到同基因小鼠的足垫中，均能致敏抗原特异性T细胞初始反应。然而，CD8α+细胞触发Th1型细胞的发育，而CD8α- DC诱导Th2型反应。这些观察结果表明，体内的Th1 / Th2平衡受初始免疫反应的抗原呈递细胞调节。
