Martínez-Pabón María Cecilia, Restrepo-Ospina Diana Patricia, Isaza-Guzmán Diana María, Orozco-Rojas Luz Marina, Tobón-Arroyave Sergio Iván
POPCAD Research Group, Laboratory of Immunodetection and Bioanalysis, Faculty of Dentistry, University of Antioquia, Calle 64 No 52-59, P.O. Box: 1226, Medellín, Colombia.
Clin Oral Investig. 2008 Mar;12(1):73-81. doi: 10.1007/s00784-007-0147-7. Epub 2007 Sep 7.
The aim of the study was to determine the prevalence of Treponema denticola in saliva of periodontally diseased and healthy patients and its relationship with the periodontal status. A 16S rRNA-based polymerase chain reaction detection method was used to determine the prevalence of T. denticola in whole saliva samples from patients with chronic periodontitis (CP, n = 37), aggressive periodontitis (AgP, n = 24), and healthy subjects (n = 28). The periodontal status of each subject was assessed by criteria based on probing depth, clinical attachment loss, and extent of periodontal breakdown. Risk factors were assessed individually and adjusted for confounding using a binary logistic regression model. The results showed that the prevalence of T. denticola in CP patients was significantly higher than those in healthy and AgP subjects (P < 0.05). Odds ratio analysis revealed a positive association for CP group/T. denticola-positive and smoking/T. denticola-positive subjects. Furthermore, all clinical measurements were significantly greater (P < 0.05) for T. denticola-positive subjects compared to T. denticola-negative subjects. After binary logistic regression analysis, both T. denticola and smoking were independently and strongly associated with development of CP. It was concluded that when used in conjunction with an optimized clinical examination protocol, this assay may offer a rapid, useful, and cost-effective tool for monitoring the presence of T. denticola in noninvasive clinical samples from both healthy and diseased patients and correlating it with the amount and extent of periodontal breakdown.
本研究的目的是确定牙周病患者和健康患者唾液中齿垢密螺旋体的患病率及其与牙周状况的关系。采用基于16S rRNA的聚合酶链反应检测方法，测定慢性牙周炎患者（CP，n = 37）、侵袭性牙周炎患者（AgP，n = 24）和健康受试者（n = 28）全唾液样本中齿垢密螺旋体的患病率。根据探诊深度、临床附着丧失和牙周破坏程度的标准评估每个受试者的牙周状况。单独评估危险因素，并使用二元逻辑回归模型对混杂因素进行校正。结果显示，CP患者中齿垢密螺旋体的患病率显著高于健康受试者和AgP患者（P < 0.05）。优势比分析显示CP组/齿垢密螺旋体阳性和吸烟/齿垢密螺旋体阳性受试者之间存在正相关。此外，与齿垢密螺旋体阴性受试者相比，齿垢密螺旋体阳性受试者的所有临床测量值均显著更高（P < 0.05）。经过二元逻辑回归分析，齿垢密螺旋体和吸烟均与CP的发生独立且密切相关。得出的结论是，当与优化的临床检查方案结合使用时，该检测方法可能为监测健康和患病患者非侵入性临床样本中齿垢密螺旋体的存在，并将其与牙周破坏的程度和范围相关联，提供一种快速、有用且具有成本效益的工具。
