用于需氧和厌氧生物回收的拭子转运系统评估。
Assessment of swab transport systems for aerobic and anaerobic organism recovery.
作者信息
Perry J L
机构信息
Microbiology Section, Department of Veterans Affair Medical Center, Wichita, Kansas 67218, USA.
出版信息
J Clin Microbiol. 1997 May;35(5):1269-71. doi: 10.1128/jcm.35.5.1269-1271.1997.
Abstract
Saline suspensions of 11 aerobes and anaerobes were used to inoculate swabs from Port-A-Cul (Becton Dickinson), Culturette EZ (Becton Dickinson), and Copan Amies gel (Copan Diagnostics). Swabs were removed from transport devices at 0, 24, and 48 h postinoculation and then extracted by vortexing in 1.0 ml of saline, and organism survival was determined by quantitative plate counts. For the organisms tested, Culturette EZ allowed < 1% of the original inocula to be recovered after 24 h. Port-A-Cul was only slightly better. Recovery was best with the Copan gel-containing system. Agar gel swab systems may be useful for multipurpose transport devices.
摘要
使用11种需氧菌和厌氧菌的盐水悬液接种来自Port-A-Cul（碧迪医疗）、Culturette EZ（碧迪医疗）和科盼阿米斯凝胶（科盼诊断公司）的拭子。在接种后0、24和48小时从运输装置中取出拭子，然后在1.0毫升盐水中涡旋提取，通过定量平板计数确定微生物存活率。对于所测试的微生物，Culturette EZ在24小时后回收率低于原始接种量的1%。Port-A-Cul稍好一点。含科盼凝胶的系统回收率最佳。琼脂凝胶拭子系统可能适用于多用途运输装置。
相似文献
1
Assessment of swab transport systems for aerobic and anaerobic organism recovery.用于需氧和厌氧生物回收的拭子转运系统评估。
J Clin Microbiol. 1997 May;35(5):1269-71. doi: 10.1128/jcm.35.5.1269-1271.1997.
2
Comparison of three transport systems (Starplex StarSwab II, the new Copan Vi-Pak Amies Agar Gel collection and transport swabs, and BBL Port-A-Cul) for maintenance of anaerobic and fastidious aerobic organisms.三种用于维持厌氧和苛求需氧菌的转运系统（Starplex StarSwab II、新型Copan Vi-Pak Amies琼脂凝胶采集和转运拭子以及BBL Port-A-Cul）的比较。
J Clin Microbiol. 2001 Jan;39(1):377-80. doi: 10.1128/JCM.39.1.377-380.2001.
3
Survival of aerobic and anaerobic bacteria in purulent clinical specimens maintained in the Copan Venturi Transystem and Becton Dickinson Port-a-Cul transport systems.需氧菌和厌氧菌在Copan文丘里转运系统和Becton Dickinson Port-a-Cul转运系统中保存的脓性临床标本中的存活情况。
J Clin Microbiol. 2000 Feb;38(2):892-4. doi: 10.1128/JCM.38.2.892-894.2000.
4
Comparison of the Copan ESwab system with two Amies agar swab transport systems for maintenance of microorganism viability.科潘ESwab系统与两种用于维持微生物活力的阿氏琼脂拭子转运系统的比较。
J Clin Microbiol. 2008 May;46(5):1655-8. doi: 10.1128/JCM.02047-07. Epub 2008 Mar 19.
5
Comparison of 3 swab transport systems for direct release and recovery of aerobic and anaerobic bacteria.三种拭子转运系统用于需氧菌和厌氧菌直接释放与回收的比较
Diagn Microbiol Infect Dis. 2008 Dec;62(4):471-3. doi: 10.1016/j.diagmicrobio.2008.08.004. Epub 2008 Sep 23.
6
Comparison of two transport systems for recovery of aerobic and anaerobic bacteria from abscesses.两种用于从脓肿中回收需氧菌和厌氧菌的转运系统的比较。
J Clin Microbiol. 1987 Oct;25(10):2020-2. doi: 10.1128/jcm.25.10.2020-2022.1987.
7
Evaluation of the effects of storage in two different swab fabrics and under three different transport conditions on recovery of aerobic and anaerobic bacteria.评估在两种不同拭子织物中以及三种不同运输条件下储存对需氧菌和厌氧菌复苏的影响。
J Clin Microbiol. 1999 Sep;37(9):3041-3. doi: 10.1128/JCM.37.9.3041-3043.1999.
8
Quantitative survival of aerobic and anaerobic microorganisms in Port-A-Cul and Copan transport systems.Port-A-Cul和Copan运输系统中需氧和厌氧微生物的定量存活情况。
J Clin Microbiol. 2008 Aug;46(8):2739-44. doi: 10.1128/JCM.00161-08. Epub 2008 Jun 25.
9
Evaluation of four commercial transport media for the survival of Neisseria gonorrhoeae.四种用于淋病奈瑟菌存活的商用运送培养基的评估
Diagn Microbiol Infect Dis. 2000 Mar;36(3):163-8. doi: 10.1016/s0732-8893(99)00134-0.
10
Comparison of direct inoculation and Copan transport systems for isolation of Neisseria gonorrhoeae from endocervical specimens.用于从宫颈标本中分离淋病奈瑟菌的直接接种法与Copan转运系统的比较。
J Clin Microbiol. 1999 Nov;37(11):3583-5. doi: 10.1128/JCM.37.11.3583-3585.1999.
引用本文的文献
1
Advances in the Sensing and Treatment of Wound Biofilms.伤口生物膜传感与治疗的进展
Angew Chem Weinheim Bergstr Ger. 2022 Mar 21;134(13):e202112218. doi: 10.1002/ange.202112218. Epub 2022 Feb 3.
2
A rapid and standardized workflow for functional assessment of bacterial biosensors in fecal samples.一种用于粪便样本中细菌生物传感器功能评估的快速且标准化的工作流程。
Front Bioeng Biotechnol. 2022 Aug 22;10:859600. doi: 10.3389/fbioe.2022.859600. eCollection 2022.
3
Advances in the Sensing and Treatment of Wound Biofilms.伤口生物膜的传感与治疗新进展。
Angew Chem Int Ed Engl. 2022 Mar 21;61(13):e202112218. doi: 10.1002/anie.202112218. Epub 2022 Feb 3.
4
16S rRNA Gene Sequence-Based Identification of Bacteria in Automatically Incubated Blood Culture Materials from Tropical Sub-Saharan Africa.基于16S rRNA基因序列对撒哈拉以南非洲热带地区自动培养血培养材料中的细菌进行鉴定
PLoS One. 2015 Aug 13;10(8):e0135923. doi: 10.1371/journal.pone.0135923. eCollection 2015.
5
Elevator buttons as unrecognized sources of bacterial colonization in hospitals.医院中未被识别的细菌定植源——电梯按钮
Open Med. 2014 Jul 8;8(3):e81-6. eCollection 2014.
6
Nasal screening for MRSA: different swabs--different results!耐甲氧西林金黄色葡萄球菌的鼻腔筛查：不同拭子——不同结果！
PLoS One. 2014 Oct 29;9(10):e111627. doi: 10.1371/journal.pone.0111627. eCollection 2014.
7
Some are more equal--a comparative study on swab uptake and release of bacterial suspensions.有些更具等效性——细菌悬液拭子吸收与释放的比较研究
PLoS One. 2014 Jul 10;9(7):e102215. doi: 10.1371/journal.pone.0102215. eCollection 2014.
8
Comparison of FecalSwab and ESwab devices for storage and transportation of Diarrheagenic bacteria.用于储存和运输致泻性细菌的粪便拭子和ESwab装置的比较
J Clin Microbiol. 2014 Jul;52(7):2334-9. doi: 10.1128/JCM.00539-14. Epub 2014 Apr 16.
9
Bacteriology swabs in primary total knee arthroplasty.初次全膝关节置换术中的细菌学拭子检查
GMS Hyg Infect Control. 2013 Apr 29;8(1):Doc02. doi: 10.3205/dgkh000202. eCollection 2013.
10
Environmental contamination by carbapenem-resistant Enterobacteriaceae.耐碳青霉烯类肠杆菌科的环境污染。
J Clin Microbiol. 2013 Jan;51(1):177-81. doi: 10.1128/JCM.01992-12. Epub 2012 Oct 31.
Zotero 插件