Samuel A D, Pitta T P, Ryu W S, Danese P N, Leung E C, Berg H C
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9863-6. doi: 10.1073/pnas.96.17.9863.
Bacteriophage chi is known to infect motile strains of enteric bacteria by adsorbing randomly along the length of a flagellar filament and then injecting its DNA into the bacterial cell at the filament base. Here, we provide evidence for a "nut and bolt" model for translocation of phage along the filament: the tail fiber of chi fits the grooves formed by helical rows of flagellin monomers, and active flagellar rotation forces the phage to follow the grooves as a nut follows the threads of a bolt.
已知噬菌体χ通过沿鞭毛丝的长度随机吸附来感染肠道细菌的运动菌株,然后将其DNA注入鞭毛丝基部的细菌细胞中。在这里,我们提供了噬菌体沿鞭毛丝易位的“螺母和螺栓”模型的证据:噬菌体χ的尾丝适合由鞭毛蛋白单体的螺旋排形成的凹槽,并且活跃的鞭毛旋转迫使噬菌体沿着凹槽移动,就像螺母沿着螺栓的螺纹移动一样。