Rubes J, Vozdová M, Kubícková S
Veterinary Research Institute, Brno, Czech Republic.
Cytogenet Cell Genet. 1999;85(3-4):200-4. doi: 10.1159/000015293.
The objective of this research was to develop chromosome-specific probes for use in evaluating aneuploidy in boar spermatozoa through the application of fluorescence in situ hybridization (FISH) technology. A multicolor FISH method was developed to detect aneuploidy in the sperm of boars using DNA probes specific for small regions of chromosomes 1, 10, and Y. The average frequencies of sperm with disomy for chromosomes 1, 10, and Y were 0.075%, 0.067%, and 0.094%, respectively. The incidence of disomy did not differ significantly by chromosome. The average frequencies of diploidy were 0.177% for 1-1-10-10 and 0.022% for Y-Y-10-10. Thus, the incidence of overall diploidy (1-1-10-10) was significantly higher than that of disomy for the chromosomes examined (P < 0.01 for disomy of the autosomes and P < 0.05 for disomy of the Y chromosome). No significant age or breed effects on disomy and diploidy rates and no significant interindividual variations in disomy or diploidy were found. The observed level of numerical chromosome aberrations in pig sperm appear to be within the range of the baseline frequencies reported so far in men.
本研究的目的是开发染色体特异性探针，通过应用荧光原位杂交（FISH）技术来评估公猪精子中的非整倍体。开发了一种多色FISH方法，使用针对1号、10号染色体和Y染色体小区域的DNA探针来检测公猪精子中的非整倍体。1号、10号和Y染色体二体精子的平均频率分别为0.075%、0.067%和0.094%。不同染色体的二体发生率无显著差异。1-1-10-10型二倍体的平均频率为0.177%，Y-Y-10-10型二倍体的平均频率为0.022%。因此，总体二倍体（1-1-10-10）的发生率显著高于所检测染色体的二体发生率（常染色体二体P < 0.01，Y染色体二体P < 0.05）。未发现年龄或品种对公猪精子二体和二倍体率有显著影响，也未发现个体间二体或二倍体有显著差异。猪精子中观察到的染色体数目畸变水平似乎在迄今为止报道的男性基线频率范围内。
