使用微通道板探测器对放射性标记凝胶进行快速、高灵敏度成像。
Rapid, high-sensitivity imaging of radiolabeled gels with microchannel plate detectors.
作者信息
Lees J E, Richards P G
机构信息
Space Research Centre, University of Leicester, UK.
出版信息
Electrophoresis. 1999 Jul;20(10):2139-43. doi: 10.1002/(SICI)1522-2683(19990701)20:10<2139::AID-ELPS2139>3.0.CO;2-R.
Abstract
A microchannel plate detector has been used to image tritium-labeled protein on one- and two-dimensional electrophoresis gels. The good spatial resolution (70 microns) and high sensitivity (6.0 dpm/mm2) of the imaging system allows detection of low levels (femto moles) of labelled proteins. We are currently using the detector for identification of new targets involved in organophosphate neurotoxicity.
摘要
一种微通道板探测器已被用于对一维和二维电泳凝胶上的氚标记蛋白质进行成像。该成像系统良好的空间分辨率（70微米）和高灵敏度（6.0每分钟衰变数/平方毫米）能够检测到低水平（飞摩尔）的标记蛋白质。我们目前正在使用该探测器来识别有机磷酸酯神经毒性中涉及的新靶点。
Zotero 插件