一维凝胶冷冻后二维（2-D）凝胶电泳质量下降。
Diminished quality of two-dimensional (2-D) gel electrophoresis after freezing of the first-dimension gels.
作者信息
Litteria M
机构信息
Neuroendocrine Research Laboratory, Veterans Affairs Medical Center, North Chicago, Illinois 60064.
出版信息
Appl Theor Electrophor. 1990;1(5):265-6.
PMID:2099182
Abstract
Two-dimensional (2-D) gels were compared before and after freezing mini isoelectric focusing (IEF) gels in their glass tubes at -20 degrees C. Overnight storage of mini IEF gels significantly reduced the separation, resolution and number of proteins in 2-D gels.
摘要
将迷你等电聚焦（IEF）凝胶在玻璃管中于-20℃冷冻前后的二维（2-D）凝胶进行了比较。迷你IEF凝胶过夜储存显著降低了二维凝胶中的分离度、分辨率和蛋白质数量。
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