选择性消除激活转录的中介蛋白突变。
Mediator protein mutations that selectively abolish activated transcription.
作者信息
Myers L C, Gustafsson C M, Hayashibara K C, Brown P O, Kornberg R D
机构信息
Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Jan 5;96(1):67-72. doi: 10.1073/pnas.96.1.67.
Abstract
Deletion of any one of three subunits of the yeast Mediator of transcriptional regulation, Med2, Pgd1 (Hrs1), and Sin4, abolished activation by Gal4-VP16 in vitro. By contrast, other Mediator functions, stimulation of basal transcription and of TFIIH kinase activity, were unaffected. A different but overlapping Mediator subunit dependence was found for activation by Gcn4. The genetic requirements for activation in vivo were closely coincident with those in vitro. A whole genome expression profile of a Deltamed2 strain showed diminished transcription of a subset of inducible genes but only minor effects on "basal" transcription. These findings make an important connection between transcriptional activation in vitro and in vivo, and identify Mediator as a "global" transcriptional coactivator.
摘要
酵母转录调控中介体（Mediator）的三个亚基Med2、Pgd1（Hrs1）和Sin4中的任何一个缺失，都会在体外消除Gal4-VP16介导的激活作用。相比之下，中介体的其他功能，即对基础转录和TFIIH激酶活性的刺激，并未受到影响。对于Gcn4介导的激活作用，发现了不同但重叠的中介体亚基依赖性。体内激活的遗传需求与体外的密切一致。Deltamed2菌株的全基因组表达谱显示，一组可诱导基因的转录减少，但对“基础”转录的影响较小。这些发现建立了体外和体内转录激活之间的重要联系，并将中介体鉴定为一种“全局”转录共激活因子。
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本文引用的文献
1
NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription.NAT是一种含有Srb多肽的人类复合物，其作为活化转录的负调节因子发挥作用。
Mol Cell. 1998 Aug;2(2):213-22. doi: 10.1016/s1097-2765(00)80131-8.
2
Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways.哺乳动物转录调控介质及其作为信号转导途径终点的可能作用。
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8538-43. doi: 10.1073/pnas.95.15.8538.
3
Transcription activation via enhanced preinitiation complex assembly in a human cell-free system lacking TAFIIs.在缺乏TAFIIs的人无细胞系统中，通过增强前起始复合物组装实现转录激活。
Mol Cell. 1998 May;1(6):925-31. doi: 10.1016/s1097-2765(00)80092-1.
4
An activator target in the RNA polymerase II holoenzyme.RNA聚合酶II全酶中的一个激活子靶点。
Mol Cell. 1998 May;1(6):895-904. doi: 10.1016/s1097-2765(00)80088-x.
5
Genetics of transcriptional regulation in yeast: connections to the RNA polymerase II CTD.酵母转录调控的遗传学：与RNA聚合酶II CTD的联系
Annu Rev Cell Dev Biol. 1997;13:1-23. doi: 10.1146/annurev.cellbio.13.1.1.
6
The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain.酵母的中介蛋白及其通过RNA聚合酶II羧基末端结构域的功能。
Genes Dev. 1998 Jan 1;12(1):45-54. doi: 10.1101/gad.12.1.45.
7
The yeast HRS1 gene is involved in positive and negative regulation of transcription and shows genetic characteristics similar to SIN4 and GAL11.酵母HRS1基因参与转录的正调控和负调控，并表现出与SIN4和GAL11相似的遗传特征。
Genetics. 1997 Dec;147(4):1585-94. doi: 10.1093/genetics/147.4.1585.
8
Exploring the metabolic and genetic control of gene expression on a genomic scale.在基因组规模上探索基因表达的代谢和遗传控制。
Science. 1997 Oct 24;278(5338):680-6. doi: 10.1126/science.278.5338.680.
9
Yeast microarrays for genome wide parallel genetic and gene expression analysis.用于全基因组平行遗传和基因表达分析的酵母微阵列。
Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13057-62. doi: 10.1073/pnas.94.24.13057.
10
Yeast TAF(II)145 functions as a core promoter selectivity factor, not a general coactivator.酵母TAF(II)145作为核心启动子选择性因子，而非一般的共激活因子发挥作用。
Cell. 1997 Aug 22;90(4):615-24. doi: 10.1016/s0092-8674(00)80523-1.
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