Shchepinov M S, Mir K U, Elder J K, Frank-Kamenetskii M D, Southern E M
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK and.
Nucleic Acids Res. 1999 Aug 1;27(15):3035-41. doi: 10.1093/nar/27.15.3035.
DNA dendrimers with two, three, six, nine or 27 arms were reassociated as complementary pairs in solution or with an array of complementary oligonucleotides on a solid support. In all cases, duplex stabilities were greater than those of unbranched molecules of equal length. A theoretical treatment for the process of dissociation of dendrimers explains the major properties of the complexes. The favourable features of DNA dendrimers-their enhanced stability and the simple predictability of their association behaviour-makes them promising as building blocks for the 'bottom up' approach to nano-assembly. These features also suggest applications in oligonucleotide array/DNA chip technology when higher hybridisation temperatures are required, for example, to melt secon-dary structure in the target.
具有两条、三条、六条、九条或二十七条臂的DNA树枝状大分子在溶液中或与固体支持物上的一系列互补寡核苷酸重新结合形成互补对。在所有情况下，双链体稳定性均高于等长的非分支分子。对树枝状大分子解离过程的理论处理解释了复合物的主要性质。DNA树枝状大分子的有利特性——增强的稳定性及其结合行为的简单可预测性——使其有望成为纳米组装“自下而上”方法的构建模块。这些特性还表明，在需要更高杂交温度（例如，用于解开靶标中的二级结构）时，可应用于寡核苷酸阵列/DNA芯片技术。
