大量相关化合物的平行合成与分析：在寡核苷酸中的应用

Parallel synthesis and analysis of large numbers of related chemical compounds: applications to oligonucleotides.

作者信息

Southern E M, Maskos U

机构信息

Department of Biochemistry, University of Oxford, UK.

出版信息

J Biotechnol. 1994 Jun 30;35(2-3):217-27. doi: 10.1016/0168-1656(94)90037-x.

DOI:10.1016/0168-1656(94)90037-x

PMID:7765059

Abstract

This review presents methods for addressing the issue of molecular complexity in biological systems. Biomolecules immobilised on solid phases can be used to probe biological interactions. As a specific example, arrays of large numbers of oligonucleotides allow the analysis of DNA sequences and complex populations of DNA molecules. The specific embodiments of this new method are presented, research up to mid-1992 outlined and requirements for future development discussed.

摘要

本综述介绍了处理生物系统中分子复杂性问题的方法。固定在固相上的生物分子可用于探测生物相互作用。作为一个具体例子，大量寡核苷酸阵列可用于分析DNA序列和复杂的DNA分子群体。文中给出了这种新方法的具体实施方案，概述了截至1992年年中的研究情况，并讨论了未来发展的要求。