Yanaura S, Yamatake Y, Ouchi T
Jpn J Pharmacol. 1976 Jun;26(3):301-8. doi: 10.1254/jjp.26.301.
A quantitative method for measuring pain threshold by the use of ultrasonic stimulation in mice has been designed. The method had the advantage of precision, simplicity of technique, rapidity of measurement, and the fact that the stimuli is innocuous upon repeated application. The nature of the senstaions induced by ultrasonic stimulus is somewhat like that felt with a prick type of pain. Pentazocine (30, 100, 150 mg/kg i.p.) aminopyrine (15,50, 100, 150 mg/kg i.p.), phenacetin (100,150, 200, 250 mg/kg i.p.) sodium salicylate (150, 200, 250 mg/kg i.p.) and other antipyretic analgesics were active in a wide range of doses indicating that this technique is sensitive to the narcotic antagonist and to the weak analgesics as well as to the narcotic analgesics as well as to the narcotic analgesics such as morphine (2.5, 5, 10, 15 mg/kg i.p.), codeine (10, 20, 25, 30, 50 mg/kg i.p.) and pethidine (5,10, 15, 20, 25 mg/kg i.p.). The ultrasonic method is, therefore, applicable in screening procedures when attempting to evaluate the analgesic potency of a wide variety of chemical agents.
已经设计出一种通过对小鼠使用超声刺激来测量疼痛阈值的定量方法。该方法具有精度高、技术简单、测量快速的优点，并且反复施加刺激时是无害的。超声刺激所诱发的感觉性质有点类似于针刺样疼痛所感觉到的。喷他佐辛（腹腔注射30、100、150毫克/千克）、氨基比林（腹腔注射15、50、100、150毫克/千克）、非那西丁（腹腔注射100、150、200、250毫克/千克）、水杨酸钠（腹腔注射150、200、250毫克/千克）以及其他解热镇痛药在很宽的剂量范围内都有活性，这表明该技术对麻醉性拮抗剂、弱镇痛药以及麻醉性镇痛药如吗啡（腹腔注射2.5、5、10、15毫克/千克）、可待因（腹腔注射10、20、25、30、50毫克/千克）和哌替啶（腹腔注射5、10、15、20、25毫克/千克）都敏感。因此，当试图评估多种化学药剂的镇痛效力时，超声方法适用于筛选程序。
