Snewin V A, Gares M P, Gaora P O, Hasan Z, Brown I N, Young D B
Department of Infectious Diseases and Microbiology, Imperial College School of Medicine, St. Mary's Campus, London W2 1PG, United Kingdom.
Infect Immun. 1999 Sep;67(9):4586-93. doi: 10.1128/IAI.67.9.4586-4593.1999.
Protective immunity to mycobacterial infection is incompletely understood but probably involves the coordinated interaction of multiple cell types and cytokines. With the aim of developing assays that might provide a surrogate measure of protective immunity, we have investigated the use of recombinant mycobacteria carrying luciferase reporter enzymes to assess the effectiveness of antimycobacterial immunity in model systems. Measurement of luminescence was shown to provide a rapid and simple alternative to the counting of CFU as a means of monitoring mycobacterial viability. We describe optimization of a luciferase reporter strain of Mycobacterium tuberculosis and demonstrate its application for the study of mycobacterial interactions with host cells in tissue culture and the rapid assessment of vaccine efficacy in a murine model.
对分枝杆菌感染的保护性免疫尚未被完全理解，但可能涉及多种细胞类型和细胞因子的协同相互作用。为了开发可能提供保护性免疫替代指标的检测方法，我们研究了使用携带荧光素酶报告酶的重组分枝杆菌来评估模型系统中抗分枝杆菌免疫的有效性。结果表明，测量发光可提供一种快速简便的替代方法，以替代通过计数CFU来监测分枝杆菌活力。我们描述了结核分枝杆菌荧光素酶报告菌株的优化，并展示了其在组织培养中研究分枝杆菌与宿主细胞相互作用以及在小鼠模型中快速评估疫苗效力的应用。
