Mesnage S, Weber-Levy M, Haustant M, Mock M, Fouet A
Toxines et Pathogénie Bactériennes, URA 1858, Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
Infect Immun. 1999 Sep;67(9):4847-50. doi: 10.1128/IAI.67.9.4847-4850.1999.
Bacillus anthracis, the causal agent of anthrax, synthesizes two surface layer (S-layer) proteins, EA1 and Sap, which account for 5 to 10% of total protein and are expressed in vivo. A recombinant B. anthracis strain was constructed by integrating into the chromosome a translational fusion harboring the DNA fragments encoding the cell wall-targeting domain of the S-layer protein EA1 and tetanus toxin fragment C (ToxC). This construct was expressed under the control of the promoter of the S-layer component gene. The hybrid protein was stably expressed on the cell surface of the bacterium. Mice were immunized with bacilli of the corresponding strain, and the hybrid protein elicited a humoral response to ToxC. This immune response was sufficient to protect mice against tetanus toxin challenge. Thus, the strategy developed in this study may make it possible to generate multivalent live veterinary vaccines, using the S-layer protein genes as a cell surface display system.
炭疽芽孢杆菌是炭疽病的病原体，它能合成两种表层（S层）蛋白，即EA1和Sap，这两种蛋白占总蛋白的5%至10%，且在体内表达。通过将一个翻译融合体整合到染色体中构建了一株重组炭疽芽孢杆菌，该融合体包含编码S层蛋白EA1细胞壁靶向结构域的DNA片段和破伤风毒素片段C（ToxC）。此构建体在S层成分基因的启动子控制下表达。杂交蛋白在细菌细胞表面稳定表达。用相应菌株的杆菌对小鼠进行免疫，杂交蛋白引发了针对ToxC的体液反应。这种免疫反应足以保护小鼠免受破伤风毒素攻击。因此，本研究中开发的策略可能使利用S层蛋白基因作为细胞表面展示系统来生产多价活兽用疫苗成为可能。
