Chuang W Y, Young T H, Yao C H, Chiu W Y
Institute of Materials Science and Engineering, College of Engineering, National Taiwan University, Taipei.
Biomaterials. 1999 Aug;20(16):1479-87. doi: 10.1016/s0142-9612(99)00054-x.
In this work, the properties of poly(vinyl alcohol) (PVA) and PVA/chitosan blended membranes were investigated by scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and electron spectroscopy for chemical analysis (ESCA). The SEM photographs show the PVA/chitosan blended membrane undergoes dramatic changes on the surface and bulk structure during the membrane formation. The DSC analysis shows that PVA and chitosan are not very compatible in the PVA/chitosan blended membrane, whereas the combination of two polymer chains of constitutionally different features is revealed. In addition, the surface of the PVA/chitosan blended membrane is enriched with nitrogen atoms at the ESCA analysis. These reflect the PVA membrane can be modified by blending with chitosan that in turn may affect the biocompatibility of the blended membrane. Therefore, adhesion and growth of fibroblasts on the PVA as well as PVA/chitosan blended membranes were investigated. Cell morphologies on the membranes were examined by SEM and cell viability was studied using MTT assay. It was observed that the PVA/chitosan blended membrane was more favorable for the cell culture than the pure PVA membrane. Cells cultured on the PVA/chitosan blended membrane had good spreading, cytoplasm webbing and flattening and were more compacting than on the pure PVA membrane. Consequently, the PVA/chitosan blended membrane may spatially mediate cellular response that can promote cell attachment and growth, indicating the PVA/chitosan blended membrane should be useful as a biomaterial for cell culture.
在本研究中，通过扫描电子显微镜（SEM）、差示扫描量热法（DSC）和化学分析电子能谱（ESCA）对聚乙烯醇（PVA）及PVA/壳聚糖共混膜的性能进行了研究。SEM照片显示，在膜形成过程中，PVA/壳聚糖共混膜的表面和整体结构发生了显著变化。DSC分析表明，PVA和壳聚糖在PVA/壳聚糖共混膜中相容性不太好，但揭示了两种具有不同结构特征的聚合物链的结合。此外，ESCA分析表明，PVA/壳聚糖共混膜表面富含氮原子。这些结果表明，PVA膜可通过与壳聚糖共混进行改性，这反过来可能会影响共混膜的生物相容性。因此，研究了成纤维细胞在PVA以及PVA/壳聚糖共混膜上的黏附与生长情况。通过SEM观察膜上的细胞形态，并使用MTT法研究细胞活力。结果发现，PVA/壳聚糖共混膜比纯PVA膜更有利于细胞培养。在PVA/壳聚糖共混膜上培养的细胞具有良好的铺展、细胞质成网和平展现象，且比在纯PVA膜上培养的细胞更加致密。因此，PVA/壳聚糖共混膜可能在空间上介导细胞反应，促进细胞附着和生长，这表明PVA/壳聚糖共混膜作为细胞培养的生物材料应该是有用的。
