School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 38541, Republic of Korea; Research Institute of Cell Culture, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 38541, Republic of Korea.
School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan, Gyeongbuk 38541, Republic of Korea.
Int J Biol Macromol. 2021 Nov 1;190:792-800. doi: 10.1016/j.ijbiomac.2021.09.034. Epub 2021 Sep 11.
Multifunctional blend membranes composed of poly (vinyl alcohol) (PVA) and fungal mushroom-derived carboxymethyl chitosan (F-CMCS) were produced using a simple solution casting technique for wound dressing applications. The structural interactions between PVA and F-CMCS were confirmed by Fourier infrared spectroscopy. The crystallinity of the membranes was examined by X-ray diffraction. Field emission scanning electron microscopy confirmed the homogeneity and coarser texture with a porous-like network in the internal structure of the membranes. The hydrophilicity, swelling, and degradation of the fabricated membranes were examined according to the F-CMCS content. The PVA/F-CMCS membrane displayed potential antibacterial activity against Escherichia coli (gram-negative) and Staphylococcus (gram-positive) bacteria. An in vitro cell study of skin fibroblasts and keratinocytes on the PVA/F-CMCS membranes confirmed the biocompatibility. The hemolysis assay demonstrated the hemocompatibility of the developed membranes. The antibacterial, biocompatibility, and good hemolysis in the PVA membrane were influenced by the F-CMCS composition ratio up to 40%. The all-inclusive properties of the PVA/F-CMCS membranes highlight its potential use in wound dressing applications.
采用简单的溶液浇铸技术制备了由聚（聚乙烯醇）（PVA）和真菌蘑菇衍生的羧甲基壳聚糖（F-CMCS）组成的多功能共混膜，用于伤口敷料应用。傅里叶变换红外光谱证实了 PVA 和 F-CMCS 之间的结构相互作用。通过 X 射线衍射研究了膜的结晶度。场发射扫描电子显微镜证实了膜的均匀性和更粗糙的纹理，其内部结构具有多孔状网络。根据 F-CMCS 的含量，对制备的膜的亲水性、溶胀性和降解性进行了研究。PVA/F-CMCS 膜对革兰氏阴性的大肠杆菌（Escherichia coli）和革兰氏阳性的金黄色葡萄球菌（Staphylococcus）表现出潜在的抗菌活性。在皮肤成纤维细胞和角质细胞上的体外细胞研究证实了 PVA/F-CMCS 膜的生物相容性。溶血试验证明了所开发膜的血液相容性。PVA 膜的抗菌性、生物相容性和良好的溶血性能受 F-CMCS 组成比例的影响高达 40%。PVA/F-CMCS 膜的综合性能突出了其在伤口敷料应用中的潜在用途。
