Pfeiffer H, Hühne J, Ortmann C, Waterkamp K, Brinkmann B
Institute of Forensic Medicine, University of Münster, von Esmarch Strasse 62, D-48149 Münster, Germany.
Int J Legal Med. 1999;112(5):287-90. doi: 10.1007/s004140050251.
The analysis of mitochondrial DNA (mtDNA) from shed hairs has gained high importance in forensic casework since telogen hairs are one of the most common types of evidence left at the crime scene. In this systematic study of hair shafts from 20 individuals, the correlation of mtDNA recovery with hair morphology (length, diameter, volume, colour), with sex, and with body localisation (head, armpit, pubis) was investigated. The highest average success rate of hypervariable region 1 (HV 1) sequencing was found in head hair shafts (75%) followed by pubic (66%) and axillary hair shafts (52%). No statistically significant correlation between morphological parameters or sex and the success rate of sequencing was found. MtDNA sequences of buccal cells, head, pubic and axillary hair shafts did not show intraindividual differences. Heteroplasmic base positions were observed neither in the hair shafts nor in control samples of buccal cells.
由于休止期毛发是犯罪现场遗留的最常见证据类型之一，因此从脱落毛发中分析线粒体DNA（mtDNA）在法医案件工作中具有高度重要性。在这项对20名个体毛发杆的系统研究中，调查了mtDNA回收率与毛发形态（长度、直径、体积、颜色）、性别以及身体部位（头部、腋窝、耻骨）之间的相关性。在头部毛发杆中发现高变区1（HV 1）测序的平均成功率最高（75%），其次是耻骨毛发（66%）和腋窝毛发杆（52%）。未发现形态学参数或性别与测序成功率之间存在统计学上的显著相关性。颊细胞、头部、耻骨和腋窝毛发杆的mtDNA序列未显示个体内差异。在毛发杆和颊细胞对照样本中均未观察到异质性碱基位置。
