Szibor Reinhard, Plate Ines, Schmitter Herrmann, Wittig Holger, Krause Dieter
Institut für Rechtsmedizin, Otto-von-Guericke-Universität Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany.
Int J Legal Med. 2006 Nov;120(6):372-6. doi: 10.1007/s00414-006-0085-y. Epub 2006 Apr 1.
At the forensic autopsy of a sexual murder victim, some trace hairs, possibly belonging to the perpetrator, were saved. Initially, the analysis of a pubic hair shaft only revealed the presence of the mitochondrial (mt) DNA haplotype profile consisting of the (CA)(6) allele and the complete hypervariable region 1 (HV1) and 2 (HV2) sequence. Later, typing of some further telogene trace hairs, which had been stored for several years, yielded a nuclear short tandem repeat (STR) profile. We used both the mtDNA haplotype and the STR profile to start a DNA mass screening project involving 2,335 male citizens of the relevant communities. MtDNA screening was carried out by using the CA repeat amplification in combination with an SNP typing procedure based on the restriction site analysis of amplified d-loop sequences. The aim of our paper is to put mass screening with mtDNA up for discussion.
在对一名性谋杀受害者进行法医尸检时，保存了一些可能属于犯罪者的微量毛发。最初，对一根阴毛干的分析仅揭示了由（CA）（6）等位基因以及完整的高变区1（HV1）和高变区2（HV2）序列组成的线粒体（mt）DNA单倍型谱。后来，对一些保存了数年的远端毛发进行分型，得到了一个核短串联重复序列（STR）谱。我们利用mtDNA单倍型和STR谱启动了一个DNA大规模筛查项目，涉及相关社区的2,335名男性公民。mtDNA筛查是通过使用CA重复扩增结合基于扩增的d环序列限制性位点分析的单核苷酸多态性（SNP）分型程序进行的。本文的目的是将mtDNA大规模筛查提出来供讨论。
