白细胞介素-4调节角膜细胞和角膜上皮细胞中由肿瘤坏死因子-α诱导产生的趋化因子。
IL-4 regulates chemokine production induced by TNF-alpha in keratocytes and corneal epithelial cells.
作者信息
Takano Y, Fukagawa K, Shimmura S, Tsubota K, Oguchi Y, Saito H
机构信息
Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
出版信息
Br J Ophthalmol. 1999 Sep;83(9):1074-6. doi: 10.1136/bjo.83.9.1074.
BACKGROUND
Eosinophils are thought to play a major role in the pathogenesis of corneal lesions in ocular allergies. The regulation of chemokine production in corneal cells by the Th2 cytokine, interleukin 4 (IL-4), was examined in order to investigate its role in ocular allergies.
METHODS
Pure cultures of human corneal epithelial cells and keratocytes were exposed to tumour necrosis factor alpha (TNF-alpha) and/or IL-4. 24 hours after exposure, culture supernatants were removed and concentrations of IL-8 and RANTES were quantified by ELISA assay.
RESULTS
Simultaneous addition of IL-4 inhibited TNF-alpha induced IL-8 production in both corneal epithelial cells and keratocytes. TNF-alpha and IL-4 synergistically stimulated the production of RANTES in keratocytes.
CONCLUSION
Differential regulation of chemokine production from corneal cells by IL-4 may play a role in the selective recruitment of predominantly eosinophils to the ocular surface in ocular allergies.
背景
嗜酸性粒细胞被认为在眼部过敏的角膜病变发病机制中起主要作用。为了研究Th2细胞因子白细胞介素4（IL-4）在眼部过敏中的作用，检测了其对角膜细胞趋化因子产生的调节作用。
方法
将人角膜上皮细胞和角膜细胞的纯培养物暴露于肿瘤坏死因子α（TNF-α）和/或IL-4。暴露24小时后，去除培养上清液，通过ELISA测定法对IL-8和RANTES的浓度进行定量。
结果
同时添加IL-4可抑制角膜上皮细胞和角膜细胞中TNF-α诱导的IL-8产生。TNF-α和IL-4协同刺激角膜细胞中RANTES的产生。
结论
IL-4对角膜细胞趋化因子产生的差异调节可能在眼部过敏中主要嗜酸性粒细胞向眼表的选择性募集过程中发挥作用。
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